Tolerance of Yarrowia lipolytica to inhibitors commonly found in lignocellulosic hydrolysates

BMC Microbiology

article

Konzock, Oliver¹  Zaghen, Simone¹  Norbeck, Joakim¹ 

[1] Department of Biology and Biological Engineering, division of Systems and Synthetic Biology, Chalmers University of Technology

关键词: Yarrowia lipolytica;    Lignocellulosic biomass;    Inhibitor tolerance;    Xylose utilization;    Furfural;    HMF;    Cinnamic acid;    Coniferyl aldehyde;    Formic acid;    Acetic acid;   

DOI  :  10.1186/s12866-021-02126-0

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

Lignocellulosic material is a suitable renewable carbon and energy source for microbial cell factories, such as Yarrowia lipolytica. To be accessible for microorganisms, the constituent sugars need to be released in a hydrolysis step, which as a side effect leads to the formation of various inhibitory compounds. However, the effects of these inhibitory compounds on the growth of Y. lipolytica have not been thoroughly investigated. Here we show the individual and combined effect of six inhibitors from three major inhibitor groups on the growth of Y. lipolytica. We engineered a xylose consuming strain by overexpressing the three native genes XR, XDH, and XK and found that the inhibitor tolerance of Y. lipolytica is similar in glucose and in xylose. Aromatic compounds could be tolerated at high concentrations, while furfural linearly increased the lag phase of the cultivation, and hydroxymethylfurfural only inhibited growth partially. The furfural induced increase in lag phase can be overcome by an increased volume of inoculum. Formic acid only affected growth at concentrations above 25 mM. In a synthetic hydrolysate, formic acid, furfural, and coniferyl aldehyde were identified as the major growth inhibitors. We showed the individual and combined effect of inhibitors found in hydrolysate on the growth of Y. lipolytica. Our study improves understanding of the growth limiting inhibitors found in hydrolysate and enables a more targeted engineering approach to increase the inhibitor tolerance of Y. lipolytica. This will help to improve the usage of Y. lipolytica as a sustainable microbial cell factory.

【 授权许可】

CC BY|CC0