Diversity patterns of soil microbial communities in the Sophora flavescens rhizosphere in response to continuous monocropping

BMC Microbiology

article

Lei, Haiying¹  Liu, Ake¹  Hou, Qinwen¹  Zhao, Qingsong¹  Guo, Jia¹  Wang, Zhijun² 

[1] Faculty of Biology Sciences and Technology, Changzhi University;Department of Chemistry, Changzhi University

关键词: Sophora flavescens;    Continuous monocropping;    Rhizosphere soil;    Microbial community;    Soil physicochemical property;   

DOI  :  10.1186/s12866-020-01956-8

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

Continuous monocropping can affect the physicochemical and biological characteristics of cultivated soil. Sophora flavescens is a valuable herbal medicine and sensitive to continuous monocropping. Currently, diversity patterns of soil microbial communities in soil continuous monocropping with S. flavescens have not been extensively elucidated. In this study, comparative 16S rDNA and internal transcribed spacer (ITS) MiSeq sequencing analyses were used to examine the taxonomic community structure and microbial diversity in nonrhizosphere soil (CK) and rhizosphere soils (SCC, TCC, and FCC) sampled from fields that had undergone two, three, and five years of continuous monocropping, respectively. Among the microbial communities, a decreased abundance of Acidobacteria and increased abundances of Proteobacteria and Bacteroidetes were found with the increase in monocropping years of S. flavescens. As the continuous monocropping time increased, the diversity of the bacterial community decreased, but that of fungi increased. Redundancy analysis also showed that among the properties of the rhizosphere soil, the available phosphorus, organic matter, total nitrogen, and sucrase had the greatest impacts on the diversity of the rhizosphere microbial community. Moreover, a biomarker for S. flavescens soil was also identified using the most differentially abundant bacteria and fungi in soil samples. Our study indicates that long-term monocropping exerted great impacts on microbial community distributions and soil physicochemical properties. The relationship between microbial community and physicochemical properties of rhizosphere soil would help clarify the side effects of continuous S. flavescens monocropping. Our study may aid in uncovering the theoretical basis underlying obstacles to continuous monocropping and provide better guidance for crop production.

【 授权许可】

CC BY|CC0