A survey of RNA viruses in mosquitoes from Mozambique reveals novel genetic lineages of flaviviruses and phenuiviruses, as well as frequent flavivirus-like viral DNA forms in Mansonia

BMC Microbiology

article

Abílio, Ana Paula¹  Parreira, Ricardo³  Silva, Manuel³  Kampango, Ayubo¹  Narciso, Inácio³  Gudo, Eduardo Samo¹  das Neves, Luís Carlos Bernardo⁴  Sidat, Mohsin²  Fafetine, José Manuel⁵  de Almeida, António Paulo Gouveia³ 

[1] Instituto Nacional de Saúde (INS)-Ministry of Health (MISAU);Faculty of Medicine, Eduardo Mondlane University (UEM);Instituto de Higiene e Medicina Tropical (IHMT)/Universidade Nova de Lisboa (NOVA), and Global Health and Tropical Medicine (GHTM) Research Centre;Faculty of Veterinary Sciences Pretoria, University of Pretoria (UP);Faculty of Veterinary, Eduardo Mondlane University (UEM)

关键词: Flaviviruses;    Bunyaviruses;    Mosquitoes;    Viral DNA forms;    Phylogenetic analysis;    Mozambique;   

DOI  :  10.1186/s12866-020-01905-5

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

Mosquito-borne diseases involving arboviruses represent expanding threats to sub-Saharan Africa imposing as considerable burden to human and veterinary public health. In Mozambique over one hundred species of potential arbovirus mosquito vectors have been identified, although their precise role in maintaining such viruses in circulation in the country remains to be elucidated. The aim of this study was to screen for the presence of flaviviruses, alphaviruses and bunyaviruses in mosquitoes from different regions of Mozambique. Our survey analyzed 14,519 mosquitoes, and the results obtained revealed genetically distinct insect-specific flaviviruses, detected in multiple species of mosquitoes from different genera. In addition, smaller flavivirus-like NS5 sequences, frequently detected in Mansonia seemed to correspond to defective viral sequences, present as viral DNA forms. Furthermore, three lineages of putative members of the Phenuiviridae family were also detected, two of which apparently corresponding to novel viral genetic lineages. This study reports for the first-time novel insect-specific flaviviruses and novel phenuiviruses, as well as frequent flavivirus-like viral DNA forms in several widely known vector species. This unique work represents recent investigation of virus screening conducted in mosquitoes from Mozambique and an important contribution to inform the establishment of a vector control program for arbovirus in the country and in the region.

【 授权许可】

CC BY|CC0