Wogonin inhibits in vitro herpes simplex virus type 1 and 2 infection by modulating cellular NF-κB and MAPK pathways

BMC Microbiology

article

Chu, Ying¹  Wu, Zhiwei²  Yun, Zhihua¹  Lv, Xiaowen³  Zhang, Longfeng⁴  Fu, Xingli⁵  Song, Siwei⁶  Su, Airong⁷  Chen, Deyan²  Xu, Lianhong¹  Wang, Yongfang¹ 

[1] Clinical Laboratory, Wujin Hospital Affiliated with Jiangsu University, Wujin Clinical College of Xuzhou Medical University;Center for Public Health Research, Medical School, Nanjing University;Department of Pediatrics, Affiliated Hangzhou First People’s Hospital, Zhejiang University School of Medicine;Clinical Laboratory, Affiliated Hospital of Jiangsu University;Health Science Center, Jiangsu University;Department of Cardiology, Renmin Hospital of Wuhan University;Central Laboratory, The Second Affiliated Hospital of Nanjing Medical University

关键词: Wogonin;    Herpes simplex virus (HSV);    Antiviral activity;    Nuclear factor κB (NF-κB);    Mitogen-activated protein kinase (MAPK);   

DOI  :  10.1186/s12866-020-01916-2

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

Wogonin, a natural flavonoid-like chemical compound, exhibits anti-inflammatory, antitumor, antiviral, neuroprotective, and anxiolytic effects by modulating a variety of cellular signaling pathways including PI3K-Akt, p53, nuclear factor κB (NF-κB), mitogen-activated protein kinase (MAPK) pathways. In this study, its antiviral effect against herpes simplex virus (HSV) type 1 and 2 (HSV-1 and HSV-2) replication was investigated. Wogonin suppressed HSV-2-induced cytopathic effect (CPE) and reduced viral mRNA transcription, viral protein synthesis, and infectious virion particle titers in a dose-dependent manner. A time-of-drug-addition assay demonstrated that wogonin acted as a postentry viral inhibitor. Wogonin also significantly reduced HSV-induced NF-κB and MAPK pathway activation, which has previously been demonstrated to be important for viral replication. Our results suggest that the anti-herpes effect of wogonin may be mediated by modulation of cellular NF-κB and JNK/p38 MAPK pathways and imply that wogonin may be useful as an anti-HSV agent.

【 授权许可】

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