Immune activation of murine RAW264.7 macrophages by sonicated and alkalized paramylon from Euglena gracilis

BMC Microbiology

article

Guo, Qingqing¹  Xu, Xu¹  Wang, Jiangxin¹  Bi, Decheng¹  Wu, Mingcan¹  Yu, Boming¹  Hu, Lang¹  Liu, Chenchen¹  Gu, Liang¹  Zhu, Hui²  Lei, Anping¹ 

[1] Shenzhen Key Laboratory of Marine Bioresources and Eco-environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University;College of Food Engineering and Biotechnology, Hanshan Normal University;College of Physics and Optoelectronic Engineering, Shenzhen University

关键词: Paramylon;    Euglena gracilis;    Immune activation;    Nuclear factor-κB;    Mitogen-activated protein kinase;    Macrophages;   

DOI  :  10.1186/s12866-020-01782-y

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

Euglena is a new super health food resource that is rich in the natural polysaccharide paramylon, a linear β-1,3-glucan with various biological activities including activity on the immune system in different cell lines and animals. Despite these reports, the immune regulation mechanism of paramylon is still unclear. We investigate the signaling pathways paramylon impacts in immune macrophages. In RAW264.7 macrophages, sonicated and alkalized paramylon oligomers up-regulated inducible nitric oxide synthase (iNOS) and increased secretion of nitric oxide (NO), interleukin (IL)-6 and tumor necrosis factor (TNF)-α, in a concentration-dependent manner. In addition, paramylon activated the nuclear factor-κB(NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways and inhibiting these pathways attenuated the paramylon-induced secretion of the above immune-mediators. These results demonstrate that Euglena gracilis paramylon modulates the immune system via activation of the NF-κB and MAPK signaling pathways and thus has potential therapeutic benefits.

【 授权许可】

CC BY|CC0