Metabolic diversity and co-occurrence of multiple Ferrovum species at an acid mine drainage site

BMC Microbiology

article

Grettenberger, Christen L.¹  Havig, Jeff R.²  Hamilton, Trinity L.³ 

[1]Department of Earth and Planetary Sciences, University of California

[2]Department of Earth and Environmental Sciences, University of Minnesota

[3]Department of Plant and Microbial Biology, University of Minnesota

[4]The BioTechnology Institute, University of Minnesota

关键词: Ferrovum;    Carbon fixation;    Iron oxidation;    Metagenome;    Acidophlic;    Biofilm;    Microcosm;    Co-occurrence;    Nitrogen;    Denoised sequence variants;   

DOI  :  10.1186/s12866-020-01768-w

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

Ferrovum spp. are abundant in acid mine drainage sites globally where they play an important role in biogeochemical cycling. All known taxa in this genus are Fe(II) oxidizers. Thus, co-occurring members of the genus could be competitors within the same environment. However, we found multiple, co-occurring Ferrovum spp. in Cabin Branch, an acid mine drainage site in the Daniel Boone National Forest, KY. Here we describe the distribution of Ferrovum spp. within the Cabin Branch communities and metagenome assembled genomes (MAGs) of two new Ferrovum spp. In contrast to previous studies, we recovered multiple 16S rRNA gene sequence variants suggesting the commonly used 97% cutoff may not be appropriate to differentiate Ferrovum spp. We also retrieved two nearly-complete Ferrovum spp. genomes from metagenomic data. The genomes of these taxa differ in several key ways relating to nutrient cycling, motility, and chemotaxis. Previously reported Ferrovum genomes are also diverse with respect to these categories suggesting that the genus Ferrovum contains substantial metabolic diversity. This diversity likely explains how the members of this genus successfully co-occur in Cabin Branch and why Ferrovum spp. are abundant across geochemical gradients.

【 授权许可】

CC BY|CC0