A novel gene expression system for Ralstonia eutropha based on the T7 promoter

BMC Microbiology

article

Hu, Muzi¹  Xiong, Bin²  Li, Zhongkang²  Liu, Li²  Li, Siwei²  Zhang, Chunzhi¹  Zhang, Xueli²  Bi, Changhao² 

[1] School of Biological Engineering, Dalian Polytechnic University;Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences;Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences

关键词: Ralstonia eutropha;    Cupriavidus necator;    T7 expression system;    pBBR1 plasmid;    Protein expression system;   

DOI  :  10.1186/s12866-020-01812-9

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

Ralstonia eutropha (syn. Cupriavidus necator) is a model microorganism for studying metabolism of polyhydroxyalkanoates (PHAs) and a potential chassis for protein expression due to various advantages. Although current plasmid systems of R. eutropha provide a basic platform for gene expression, the performance of the expression-inducing systems is still limited. In addition, the sizes of the cloned genes are limited due to the large sizes of the plasmid backbones. In this study, an R. eutropha T7 expression system was established by integrating a T7 RNA polymerase gene driven by the PBAD promoter into the genome of R. eutropha, as well as adding a T7 promoter into a pBBR1-derived plasmid for gene expression. In addition, the essential DNA sequence necessary for pBBR1 plasmid replication was identified, and the redundant parts were deleted reducing the expression plasmid size to 3392 bp, which improved the electroporation efficiency about 4 times. As a result, the highest expression level of RFP was enhanced, and the L-arabinose concentration for expression induction was decreased 20 times. The R. eutropha T7 expression system provides an efficient platform for protein production and synthetic biology applications.

【 授权许可】

CC BY|CC0