Viral metagenomic analysis of fecal samples reveals an enteric virome signature in irritable bowel syndrome

BMC Microbiology

article

Ansari, Mina Hojat¹  Ebrahimi, Mehregan³  Fattahi, Mohammad Reza¹  Gardner, Michael G.⁴  Safarpour, Ali Reza¹  Faghihi, Mohammad Ali⁶  Lankarani, Kamran Bagheri² 

[1] Gastroenterohepatology Research Center, Shiraz University of Medical Sciences;Health Policy Research Center, Institute of Health, Shiraz University of Medical Sciences;Department of Biology, Shiraz University;College of Science and Engineering, Flinders University;Evolutionary Biology Unit;Persian Bayan Gene Research and Training Center, Dr. Faghihi’s Medical Genetics Center;Center for Therapeutic Innovation, Department of Psychiatry and Behavioral Sciences, University of Miami Miller School of Medicine

关键词: Irritable bowel syndrome;    Microbiota;    Enteric virome;    Metagenomics analysis;    Bacteriophages;    Eukaryotic viruses;   

DOI  :  10.1186/s12866-020-01817-4

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

Changes in the enteric microbiota have been suggested to contribute to gastrointestinal diseases, including irritable bowel syndrome. Most of the published work is on bacterial dysbiosis with meager data on the role of the virome in irritable bowel syndrome and other gastrointestinal diseases. In the current study, we therefore aimed to investigate the viral community composition of the gut and test for potential dysbiosis linked to irritable bowel syndrome. A metagenomics analysis on fecal samples of 50 individuals — 30 of whom met the Rome IV criteria for IBS and 20 healthy controls— was conducted. There was a noticeable alteration in viral taxa observed in association with irritable bowel syndrome when compared to healthy individuals — where some eukaryotic viral taxa noticeably prevail over others. We observed a significant decrease in the diversity and abundance of enteric virome particularly in eukaryotic viruses of Megavirales in patients with irritable bowel syndrome. These findings shed light on a new hypothesis that the alteration of the viral taxa contributes to the pathogenesis of irritable bowel syndrome and related symptoms, and therefore, pave the way for developing a new diagnostic biomarker or anti-viral drugs for the treatment of irritable bowel syndrome.

【 授权许可】

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