Lactobacilli spp.: real-time evaluation of biofilm growth

BMC Microbiology

article

Martinez, Stacy¹  Schlabritz-Loutsevitch, Natalia¹  Garcia, Jonathan Gomez¹  Williams, Roy¹  Elmassry, Moamen⁴  West, Andrew¹  Hamood, Abdul⁵  Hurtado, Deborah⁶  Gudenkauf, Brent¹  Ventolini, Gary¹ 

[1] Texas Tech University Health Sciences Center at the Permian Basin;Department of Neurobiology and Pharmacology, Texas Tech University Health Sciences Center;University of Texas at the Permian Basin;Department of Biological Sciences, Texas Tech University;Department of Microbiology and Immunology, Texas Tech University Health Sciences Center;Inc.

关键词: Real-time detection;    Biofilm;    Lactobacilli;    Micro-fermenter;    xCELLigence;    RNA-seq;   

DOI  :  10.1186/s12866-020-01753-3

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

Biofilm is a fundamental bacterial survival mode which proceeds through three main generalized phases: adhesion, maturation, and dispersion. Lactobacilli spp. (LB) are critical components of gut and reproductive health and are widely used probiotics. Evaluation of time-dependent mechanisms of biofilm formation is important for understanding of host-microbial interaction and development of therapeutic interventions. Time-dependent LB biofilm growth was studied in two systems: large biofilm output in continuous flow system (microfermenter (M), Institute Pasteur, France) and electrical impedance-based real time label-free cell analyzer (C) (xCELLigence, ACEA Bioscience Inc., San Diego, CA). L. plantarum biofilm growth in M system was video-recorded, followed by analyses using IMARIS software (Bitplane, Oxford Instrument Company, Concord, MA, USA). Additionally, whole genome expression and analyses of attached (A) and dispersed (D) biofilm phases at 24 and 48 h were performed. The dynamic of biofilm growth of L. plantarum was similar in both systems except for D phases. Comparison of the transcriptome of A and D phases revealed, that 121 transcripts differ between two phases at 24 h. and 35 transcripts – at 48 h. of M growth. The main pathways, down-regulated in A compared to D phases after 24 h. were transcriptional regulation, purine nucleotide biosynthesis, and L-aspartate biosynthesis, and the upregulated pathways were fatty acid and phospholipid metabolism as well as ABC transporters and purine nucleotide biosynthesis. Four LB species differed in the duration and amplitude of attachment phases, while growth phases were similar. LB spp. biofilm growth and propagation area dynamic, time-dependent processes with species-specific and time specific characteristics. The dynamic of LB biofilm growth agrees with published pathophysiological data and points out that real time evaluation is an important tool in understanding growth of microbial communities.

【 授权许可】

CC BY|CC0