Insight to shape of soil microbiome during the ternary cropping system of Gastradia elata

BMC Microbiology

article

Yuan, Qing-Song¹  Kang, Chuanzhi²  Zhou, Tao¹  Xu, Jiao¹  Jiang, Weike¹  Ou, Xiaohong¹  Wang, Hui¹  Guo, Lanping²  Xiao, Chenghong¹  Wang, Yanhong¹  Wang, Xiao³ 

[1] Guizhou University of Traditional Chinese Medicine;National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences;Shandong Analysis and Test Center, Shandong Academic of Sciences

关键词: Gastradia elata;    Armillaria mella;    Microbiome;    Rhizosphere soil;    Mycorrhizosphere soil;    Ternary cropping system;    Next generation pyrosequencing;   

DOI  :  10.1186/s12866-020-01790-y

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

The ternary cropping system of Gastradia elata depends on a symbiotic relationship with the mycorrhizal fungi Armillaria mellea, which decays wood to assimilate nutrition for the growth of G. elata. The composition of microbe flora as key determinants of rhizoshere and mycorrhizoshere soil fertility and health was investigated to understand how G. elata and A. mellea impacted on its composition. The next generation pyrosequencing analysis was applied to assess the shift of structure of microbial community in rhizoshere of G. elata and mycorrhizoshere of A. mellea compared to the control sample under agriculture process. The root-associated microbe floras were significantly impacted by rhizocompartments (including rhizoshere and mycorrhizoshere) and agriculture process. Cropping process of G. elata enhanced the richness and diversity of the microbial community in rhizoshere and mycorrhizoshere soil. Furthermore, planting process of G. elata significantly reduced the abundance of phyla Basidiomycota, Firmicutes and Actinobacteria, while increased the abundance of phyla Ascomycota, Chloroflexi, Proteobacteria, Planctomycetes, and Gemmatimonadetes in rhizoshere and mycorrhizoshere. Besides, A. mellea and G. elata significantly enriched several members of saprophytoic and pathogenic fungus (i.e., Exophiala, Leptodontidium, Cosmospora, Cercophora, Metarhizium, Ilyonectria, and Sporothrix), which will enhance the possibility of G. elata disease incidence. At the same time, the ternary cropping system significantly deterred several members of beneficial ectomycorrhizal fungus (i.e., Russula, Sebacina, and Amanita), which will reduce the ability to protect G. elata from diseases. In the ternary cropping system of G. elata, A. mellea and G. elata lead to imbalance of microbial community in rhizoshere and mycorrhizoshere soil, suggested that further studies on maintaining the balance of microbial community in A. mellea mycorrhizosphere and G. elata rhizosphere soil under field conditions may provide a promising avenue for high yield and high quality G. elata.

【 授权许可】

CC BY|CC0