The canine oral microbiome: variation in bacterial populations across different niches

BMC Microbiology

article

Ruparell, Avika¹  Inui, Taichi¹  Staunton, Ruth¹  Wallis, Corrin¹  Deusch, Oliver¹  Holcombe, Lucy J.¹ 

[1] WALTHAM Petcare Science Institute

关键词: Oral;    Canine;    Microbiome;    Plaque;    Buccal;    Tongue;    Saliva;   

DOI  :  10.1186/s12866-020-1704-3

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

Microbiota from different niches within the canine oral cavity were profiled and compared. Supragingival plaque and stimulated saliva, were collected alongside samples from the buccal and tongue dorsum mucosa, from 14 Labrador retrievers at three timepoints within a 1 month timeframe. The V3-V4 region of the 16S rRNA gene was sequenced via Illumina MiSeq. Supragingival plaque microbiota had the highest bacterial diversity and the largest number of significant differences in individual taxa when compared to the other oral niches. Stimulated saliva exhibited the highest variability in microbial composition between dogs, yet the lowest bacterial diversity amongst all the niches. Overall, the bacteria of the buccal and tongue dorsum mucosa were most similar. The bacterial community profiles indicated three discrete oral niches: soft tissue surfaces (buccal and tongue dorsum mucosa), hard tissue surface (supragingival plaque) and saliva. The ability to distinguish the niches by their microbiota signature offers the potential for microbial biomarkers to be identified in each unique niche for diagnostic use.

【 授权许可】

CC BY|CC0