Oral rinses in growth inhibition and treatment of Helicobacter pylori infection

BMC Microbiology

article

Kashyap, Dharmendra¹  Baral, Budhadev¹  Verma, Tarun Prakash¹  Sonkar, Charu¹  Chatterji, Debi²  Jain, Ajay Kumar²  Jha, Hem C.¹ 

[1] Discipline of Biosciences and Biomedical Engineering, Indian Institute of Technology Indore;Choithram Hospital and Research Centre

关键词: Gastric cancer;    Oral rinses;    CagA;    Apoptotic pathway;    Growth curve;    H;    pylori;    Gastritis;   

DOI  :  10.1186/s12866-020-01728-4

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

Helicobacter pylori (H. pylori) is well-known for its role in chronic gastritis and gastric cancer. Eradication of these carcinogenic bacteria from the gut is one of the challenges for clinicians. The complexity of treatment mainly owes to antibiotic resistance and relapse due to an additional reservoir in the oral cavity. Our study emphases the isolation of H. pylori from distinct habitats of the gut microenvironment (gastric biopsy and gastric juice) and its subsequent characterization. We have also evaluated the effect of various oral rinses on isolated H. pylori from different anatomical locations of included subjects. The possible strains isolated from two different habitats of the same subject shows a striking difference in their growth pattern. Promisingly, some of the included oral rinses are efficient in growth inhibition as per recommended 30 s treatment. The subsequent evaluation shows that oral rinse B (among A-E) is most effective and down-regulates the expression of one of the potent H. pylori gene, CagA, in the infected gastric adenocarcinoma (AGS) cells. Our study, for the first time, revealed that H. pylori, isolated from the different habitat of the same subject, show a different growth pattern. The expression of H. pylori pathogenic gene (CagA) was down-regulated by the use of oral rinses. Hence, oral rinses will reduce the H. pylori in the oral cavity and help to control its migration from oral to the gastric compartment and may be used as an adjuvant treatment option for its re-infection.

【 授权许可】

CC BY|CC0