Site Directed Mutagenesis of LuxG as a Flavin Reductase from Bioluminescent Bacteria of Photobacterium leiognathi

Journal of the Korean Chemical Society

article

Ki Seok Nam¹  Eui Ho Lee¹  Eugeny Oh¹  Chan Yong Lee¹ 

[1] Department of Biochemistry, Chungnam National University

关键词: Bioluminescence;    Flavin reductase;    LuxG;    Photobacterium;    Site-directed mutagenesis;   

DOI  :  10.5012/jkcs.2020.64.4.225

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

Riboflavin is a water-soluble vitamin that is involved in respiration physiologically, in a form of coenzyme FMN (riboflavin 5’-phosphate) and FAD (flavin adenine dinucleotide), contributing to various oxidation-reduction reactions.1 It serves the important roles of delivering and accepting of hydrogen atoms, protons, or hydride ions through several organic metabolites, such as amino acids, fatty acids, and pyrimidine.2 The core part of the isoalloxazine ring in flavin acts as a donor and acceptor for two electrons.

【 授权许可】

Unknown