Formate dehydrogenase takes part in molybdenum and iron homeostasis and affects dark-induced senescence in plants

Journal of plant interactions

article

Irene Murgia¹  Gianpiero Vigani²  Dario Di Silvestre³  Pierluigi Mauri³  Rossana Rossi³  Andrea Bergamaschi³  Miriam Frisella¹  Piero Morandini⁴ 

[1] University of Milano;b Plant Physiology Unit, Life Sciences and Systems Biology Dept., University of Torino;c Proteomic and Metabolomic Laboratory;d Environmental Science and Policy Dept., University of Milano

关键词: Arabidopsis thaliana;    dark-induced senescence;    formate dehydrogenase;    iron;    molybdenum;    Nicotiana tabacum;   

DOI  :  10.1080/17429145.2020.1836273

学科分类：纳米科学和纳米技术

来源: Taylor & Francis

【 摘 要 】

Formate is produced, in plants, by various biochemical pathways and it is degraded by Formate Dehydrogenase FDH, in presence of NAD + , into CO 2 and NADH. FDH has been proposed as one of the enzymes regulating molybdenum (Mo) and iron (Fe) homeostasis. Here we explored the impact of FDH perturbation on Mo and Fe plant nutritional status and FDH relevance on the plant responses against abiotic stresses, by using in silico and experimental approaches. The characterization of different Arabidopsis thaliana and Nicotiana tabacum FDH transgenic lines suggests that FDH promoter activity is dependent on both Mo and Fe nutritional supply and that FDH overexpression alters Mo concentrations in seeds and Fe concentration in seeds, leaves and stems. Also, FDH overexpression delays the dark-induced senescence whereas the lack of FDH accelerates its progression. FDH is therefore a multifaceted enzyme with impact on Mo and Fe homeostasis and regulation of dark-induced senescence.

【 授权许可】

CC BY|CC BY-NC