OsRuvB transgene induces salt tolerance in pigeon pea

Journal of plant interactions

article

Rakshita Singh¹  Sudhir Sharma²  Pushpa Kharb¹  Shabnam Saifi³  Narendra Tuteja³ 

[1] a Department of Molecular Biology, College of Basic Sciences and Humanities;b Stockbridge School of Agriculture, University of Massachusetts;c International Centre for Genetic Engineering and Biotechnology

关键词: Salinity stress;    OsRuvB;    transgenic pigeon pea;    induced tolerance;   

DOI  :  10.1080/17429145.2020.1722267

学科分类：纳米科学和纳米技术

来源: Taylor & Francis

【 摘 要 】

Salinity of cultivable land is a growing global concern that has been affecting the yields of major crops worldwide such as pigeon pea. In the current study, transgenic pigeon pea plants were developed using an in-planta Agrobacterium-mediated genetic transformation method wherein OsRuvB, a rice DNA helicase gene, was incorporated to induce salt tolerance in pigeon pea plants. Transformation efficiency of 35.7% was achieved with stable insertion of OsRuvB in transgenic lines. When subjected to salinity stress induced by 75 mM NaCl increase in chlorophyll content, relative water content, peroxidase and catalase activity in transgenic lines was observed over the wild type plants. Membrane injury index and lipid peroxidation were significantly reduced in transgenic lines. Proline and Total Soluble Sugar content were enhanced in both transgenic plants and wild type strains. It was inferred that transgenic lines were tolerant to salinity stress and tolerance may be imparted through an alternative unknown pathway.

【 授权许可】

CC BY|CC BY-NC