Characteristics of systemic infection and host responses in chickens experimentally infected with Salmonella enterica serovar Gallinarum biovar Gallinarum

The Journal of Veterinary Medical Science

article

Shinjiro OJIMA¹  Masashi OKAMURA¹  Nana OSAWA¹  Akiko TAMURA¹  Kazuki YOSHIOKA³  Takashige KASHIMOTO⁴  Takeshi HANEDA⁵  Hisaya K. ONO¹  Dong-Liang HU¹ 

[1] Laboratory of Zoonoses, Kitasato University School of Veterinary Medicine;Section of Applied Veterinary Sciences, Division of Veterinary Sciences, Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine;Laboratory of Veterinary Anatomy, Kitasato University School of Veterinary Medicine;Laboratory of Veterinary Public Health, Kitasato University School of Veterinary Medicine;Laboratory of Microbiology, Kitasato University School of Pharmacy

关键词: chicken;    fowl typhoid;    Salmonella Gallinarum;    systemic infection;   

DOI  :  10.1292/jvms.21-0227

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Salmonella enterica serovar Gallinarum biovar Gallinarum ( S. Gallinarum) is a host-specific pathogen causing systemic infection in poultry, which leads to significant economic losses due to high mortality. However, little is known about the dynamic process of systemic infection and pathogenic characteristics of S. Gallinarum in chickens. In the present study, we developed an oral infection model that reproduces the pathology of S. Gallinarum and clarified the host immune response of the infected chickens. Chickens at 20 days of age orally inoculated at a dose of 10 8 colony forming unit (CFU) showed typical clinical signs of fowl typhoid and died between 6 and 10 days post infection. The inoculated S. Gallinarum rapidly disseminated to multple organs and the bacterial counts increased in the liver and spleen at 3 days post infection. Pathological changes associated wirh inflammation in the liver and spleen became apparent at 4 days post infection, and increased expression of interferon (IFN)-γ and interleuikin (IL)-12 in the liver and spleen did not observed until 3 days post infection. These results indicate that S. Gallinarum rapidly spread to entire body through intestine, and the low-level of inflammatory responses in the liver during the early stage of infection may contribute to rapid, systemic dissemination of the bacteria. Our infection model and findings will contribute to the better understanding of the pathogenic mechanism of S . Gallinarum, and provide new insights into the prevention and control of fowl typhoid.

【 授权许可】

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