Validation of a newly generated oxytocin antibody for enzyme-linked immunosorbent assays

The Journal of Veterinary Medical Science

article

Kaori MURATA¹  Miho NAGASAWA¹  Tatsushi ONAKA³  Ken-ichi TAKEYAMA⁴  Takefumi KIKUSUI¹ 

[1] School of Veterinary Medicine, Azabu University;Center for Human and Animal Symbiosis Science, Azabu University;Division of Brain and Neurophysiology, Department of Physiology, Jichi Medical University;Ltd.;Research Institute Agricultural and Life Sciences, Tokyo University of Agriculture

关键词: enzyme-linked immunosorbent assay;    oxytocin;    polyclonal antibody;   

DOI  :  10.1292/jvms.20-0723

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

The biological and psychological significance of oxytocin is increasingly recognized; however, reliable assays of oxytocin in biological samples have not been developed. We raised a new oxytocin polyclonal rabbit antibody against synthetic oxytocin. The affinity of antibodies to oxytocin was examined by a radio-immunoassay and compared with that of a previously validated antibody. One antibody showed affinity for oxytocin in the radio-immunoassay. We developed a solid-phase ELISA for oxytocin using this antibody and compared it with existing methods. The newly developed ELISA showed comparable results using urine samples but not using serum samples. These results indicate that the new ELISA is useful for urinary oxytocin; further modifications, such as different extraction methods, are needed for its application to serum oxytocin.

【 授权许可】

Unknown