Genotyping of infectious bronchitis viruses isolated in Japan during 2008−2019

The Journal of Veterinary Medical Science

article

Masaji MASE¹  Makiko GOTOU³  Daisuke INOUE⁴  Satoko WATANABE¹  Hiroshi ISEKI¹ 

[1] Division of Viral Disease and Epidemiology, National Institute of Animal Health, National Agriculture and Food Research Organization;United Graduate School of Veterinary Sciences, Gifu University;Tokushima Animal Hygiene Service Center of Tokushima Prefecture;Chuo Livestock Hygiene Service Center

关键词: diversity;    genotype;    infectious bronchitis virus;   

DOI  :  10.1292/jvms.20-0620

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Seventeen isolates of infectious bronchitis virus (IBV) were obtained from various prefectures of Japan during 2008–2019 and genetically analyzed. The IBV isolates were classified into six genetic groups, based on phylogenetic analysis of the S1 gene. The S1 genotypes were distinguishable by a newly developed restriction fragment length polymorphism (RFLP) method using three endonucleases, Hae II, Hpa I, and Fok I. Moreover, the isolates were classified into four genetic groups, based on phylogenetic analysis of the S2 gene. However, novel genetic groups based on a combination of S1 and S2 genotypes, which were undetected previously, were confirmed in this study, indicating that various recombinant IBV strains were prevalent in poultry in Japan.

【 授权许可】

Unknown