Transcriptomic Features of Echinococcus granulosus Protoscolex during the Encystation Process

The journal of physiological sciences

article

Junjie Fan¹  Hongye Wu¹  Kai Li¹  Xunuo Liu¹  Qingqing Tan¹  Wenqiao Cao¹  Bo Liang²  Bin Ye¹ 

[1] Department of Pathogenic Biology, Chongqing Medical University;Chongqing No.18 Middle School

关键词: Echinococcus granulosus;    encystation process;    differentially expressed gene;    protoscolex;    transcriptome;   

DOI  :  10.3347/kjp.2020.58.3.287

来源: Springer

【 摘 要 】

Cystic echinococcosis (CE) is a zoonotic infection caused by Echinococcus granulosus larvae. It seriously affects the development of animal husbandry and endangers human health. Due to a poor understanding of the cystic fluid formation pathway, there is currently a lack of innovative methods for the prevention and treatment of CE. In this study, the protoscoleces (PSCs) in the encystation process were analyzed by high-throughput RNA sequencing. A total of 32,401 transcripts and 14,903 cDNAs revealed numbers of new genes and transcripts, stage-specific genes, and differently expressed genes. Genes encoding proteins involved in signaling pathways, such as putative G-protein coupled receptor, tyrosine kinases, and serine/threonine protein kinase, were predominantly up-regulated during the encystation process. Antioxidant enzymes included cytochrome c oxidase, thioredoxin glutathione, and glutathione peroxidase were a high expression level. Intriguingly, KEGG enrichment suggested that differentially up-regulated genes involved in the vasopressin-regulated water reabsorption metabolic pathway may play important roles in the transport of proteins, carbohydrates, and other substances. These results provide valuable information on the mechanism of cystic fluid production during the encystation process, and provide a basis for further studies on the molecular mechanisms of growth and development of PSCs.

【 授权许可】

Unknown