Validation of an UHPLC-MS/MS Method for the Determination of Malachite Green, Leucomalachite Green, Crystal Violet, and Leucocrystal Violet in Shrimp, Fish, and Salmon Muscle Using a Modified QuEChERS Approach

Journal of the Brazilian Chemical Society

article

Rocha, Diego G.¹  Souza, Leonardo F. de¹  Lana, Mary Ane G.¹  Silva, Thaís M. L. da²  Assis, Débora C. S. de² 

[1] Laboratório Federal de Defesa Agropecuária (LFDA-MG);Universidade Federal de Minas Gerais (UFMG)

关键词: crystal violet;    leucocrystal violet;    malachite green;    leucomalachite green;    QuEChERS;   

DOI  :  10.21577/0103-5053.20200224

学科分类：内科医学

来源: SciELO

【 摘 要 】

A quantitative and confirmatory method for detecting the presence of triphenylmethane dyes in shrimp muscle using ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) and a quick, easy, cheap, effective, rugged and safe (QuEChERS) extraction approach was validated. The method exhibited linearity and selectivity and the coefficient of determination (R 2 ) was higher than 0.95 for all studied analytes. Limits of detection (LODs) varied from 0.32 to 0.44 µg kg -1 and the limit of quantification (LOQ) was determined to be 0.5 µg kg -1 for all studied analytes. The trueness, precision, decision limits (CCα), detection capability (CCβ) and uncertainty presented adequate performance. In addition to the validation in shrimp muscle, fish and salmon muscle were also satisfactory validated as an extension of scope. The suitability of the proposed method was also evaluated through an interlaboratory proficiency test, in which satisfactory results were obtained. The fully validated method is thus suitable for the analysis of triphenylmethane dyes in shrimp, fish, and salmon muscle.

【 授权许可】

CC BY