Structural Study of Wheat Gliadin in Different Solvents by Spectroscopic Techniques

Journal of the Brazilian Chemical Society

article

Ribeiro, Andresa C.¹  Leite, Daiani C.¹  Scheibel, Jóice M.¹  Soares, Rosane M. D.¹  Silveira, Nádya P.¹ 

[1] Laboratório de Bio&Macromolecules, Instituto de Química, Universidade Federal do Rio Grande do Sul

关键词: gliadin;    spectroscopy;    morphology;    stability;    biotechnology;   

DOI  :  10.21577/0103-5053.20200221

学科分类：内科医学

来源: SciELO

【 摘 要 】

Physicochemical properties of gliadin in different solvents (dimethyl sulfoxide (DMSO), H 2 O, and aqueous ethanol) and pH (9.8, 6.8, and 1.2) were investigated using dynamic light scattering (DLS), zeta potential (ZP), and attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). Gliadin-DMSO and gliadin-deionized water (H 2 O) (pH 9.8) showed a lower size distribution, whereas samples solubilized in 60% aqueous ethanol presented a lower size distribution only at pH 1.2. ZP analysis showed that gliadin-H 2 O (pH 9.8) was the most stable evaluated system. ZP results of gliadin-DMSO indicated an unstable system, with the coexistence of several protein conformations. ATR-FTIR analysis showed that, in H 2 O, most protein conformations were β-sheets, while in DMSO a band at 1660 cm −1 appeared to be related to protein unfolding. The techniques proved to be effective in monitoring conformation and stability of all gliadin/solvent systems. Such information can be used in the development of new gliadin-based materials.

【 授权许可】

CC BY