Polyphenol Profile and Quantitative Assessment of the Flavonoid Kaempferitrin in Wild and Cultivated Brazilian Amazonian Uncaria guianensis (Rubiaceae)

Journal of the Brazilian Chemical Society

article

Paixão, Djavan da¹  Barboza, Rodolfo S.¹  Valente, Ligia M. M.¹  Souza, Matheus O.¹  Siani, Antonio C.²  Pereira, Rita C. A.³  Gallo, Blanca⁴  Berrueta, Luis A.⁴ 

[1] Instituto de Química, Universidade Federal do Rio de Janeiro;Instituto de Tecnologia em Fármacos;Embrapa Agroindústria Tropical;Departamento de Química Analítica, Universitad del País Vasco/Euskal Herriko Unibertsitatea (UPV/EHU)

关键词: Uncaria guianensis;    Rubiaceae;    cat’s claw;    polyphenolic compounds;    kaempferitrin;    chemical marker;   

DOI  :  10.21577/0103-5053.20210064

学科分类：内科医学

来源: SciELO

【 摘 要 】

The Amazonian Rubiaceae species Uncaria guianensis (UG) is locally used as anti-inflammatory, antitumor, antidiabetic, anti-ulcers, and others. The phenolic content of its leaves is characterized by the great predominance of the flavonoid kaempferol-3,7- O -(α)-L-dirhamnoside (kaempferitrin). The present study quantitatively evaluates the kaempferitrin content in the leaves and branches of cultivated and wild UG specimens collected in different locations of the Brazilian Amazon rainforest by employing high-performance liquid chromatography coupled to a diode array detector (HPLC-DAD). Besides, the understanding of the polyphenol profile performed by electron spray ionization is deepened by tandem mass spectrometry analysis (ESI-MS/MS), using a previously approached leaf UG extract, and the flavonoid quercetin-3,7-O-(α)-L-dirhamnoside was first isolated from UG. All samples showed quite similar qualitative polyphenol profiles. Kaempferitrin in UG ranged from 1.1 to 1.9 mg 100 mg -1 for dry leaves of adult wild plants, 0.3 to 0.7 mg 100 mg -1 for dry leaves of cultivated young plants and 0.00 to 0.04 mg 100 mg -1 for dry branches of adult wild plants. Besides suggesting the distribution of kaempferitrin in the species, these results reinforce this flavonol as a suitable chemical marker for UG leaves and the products derived from them.

【 授权许可】

CC BY