In vivo and in vitro Volatile Constituents of the Flowers of Xylopia aromatica by HS-SPME/GC-MS

Journal of the Brazilian Chemical Society

article

Junqueira, João G. M.¹  Nascimento, Michelle N. G. do¹  Costa, Lucas G. da³  Romualdo, Lincoln L.¹  Aquino, Francisco W. B. de²  Abubakar, Mustapha N.⁴  Terezan, Ana P.⁵  Cunha, Gustavo O. S.⁵  Severino, Vanessa G. P.⁵ 

[1] Instituto de Química, Universidade Federal de Catalão;Departamento de Química, Universidade Federal de São Carlos;Instituto de Química, Universidade Federal de Uberlândia;Department of Chemistry, Federal College of Education (Technical) Gusau;Instituto de Química, Universidade Federal de Goiás

关键词: Xylopia aromatica;    in vivo and in vitro sampling;    HS-SPME/GC-MS;    VOCs;    multivariate analysis;   

DOI  :  10.21577/0103-5053.20210013

学科分类：内科医学

来源: SciELO

【 摘 要 】

Xylopia aromatica (Lam.) Mart. (Annonaceae) is a typical species from the Brazilian cerrado that presents medicinal properties. The plant is distinguished by its large white flowers which produce a pleasant fragrance. X. aromatica is characterized by a wide range of medicinal application. These characteristics have motivated us to investigate the flowers volatile organic compounds (VOCs) via in vivo and in vitro protocols by a headspace solid-phase microextraction (HS-SPME) technique combined with gas chromatography-mass spectrometry (HS-SPME/GC-MS). Four different fibers, extraction times and temperatures were the parameters changed to lead to the maximum profiling of the volatile constituents. Data were analyzed using principal component analysis (PCA). A total of 77 VOCs were extracted from the floral scent, with 52 and 68 extracted from in vivo and in vitro sampling, respectively, of which 48 were reported for the first time in the literature as volatile constituents from X. aromatica flowers. The extraction and identification of VOCs were successfully performed through HS-SPME/GC-MS. The PCA data allowed the identification of parameters that led to the maximum number of VOCs, which were polyacrylate (PA) and carboxen/polydimethylsiloxane (CAR/PDMS) fibers, 60 min extraction time and temperature of 29.0 °C. Among the volatile constituents identified, sesquiterpenes predominated, comprising about 61.04%.

【 授权许可】

CC BY