Sample Preparation Using TMAH and Nitric Acid for Multielement Determination in Crustacean Samples by MIP OES

Journal of the Brazilian Chemical Society

article

Lemos, Michelle S.¹  Cruz, Allan S.¹  Dantas, Kelly G. F.¹ 

[1] Universidade Federal do Pará

关键词: tetramethylammonium hydroxide;    shrimp;    crab;    microwave induced plasma;    inorganic elements;   

DOI  :  10.21577/0103-5053.20200200

学科分类：内科医学

来源: SciELO

【 摘 要 】

This study proposes a simple sample preparation procedure using tetramethylammonium hydroxide (TMAH) and nitric acid (HNO 3 ) to determine Cr, Cu, Fe, K, Mg, Mn, and Zn concentrations in shrimp ( Macrobrachium amazonicum ) and crab ( Ucides cordatus ) samples from the Amazon region by microwave-induced plasma optical emission spectrometry (MIP OES). The sample solubilization was carried out employing TMAH and HNO 3 using an ultrasound bath and a thermostatic water bath. The accuracy of the proposed procedure was performed using microwave-assisted digestion as comparative method. Certified fish protein reference material (DORM-4) and also addition recovery experiments were used to assess the accuracy of the proposed procedure and recoveries obtained were adequate, ranging from 91 to 106%. In this study, the use of TMAH and HNO 3 simplified sample preparation, maintaining the identity of analytes and ensuring efficiency in determinations.

【 授权许可】

CC BY