In silico Characterization of the Structure of Genes and Proteins related to β-carotene Degradation in Musa acuminata ‘ DH-Pahang’ and Musa balbisiana ‘ Pisang Klutuk Wulung’

Pertanika Journal of Tropical Agricultural Science

article

Tatas H. P. Brotosudarmo¹  Karlia Meitha²  Nisrina Sukriandi²  Fenny Martha Dwivany² 

[1]Ma Chung Research Center for Photosynthetic Pigments (MRCPP) and Department of Chemistry, Universitas Ma Chung

[2]School of Life Sciences and Technology, Institut Teknologi Bandung

[3]Bali International Research Center for Banana

[4]Bioscience and Biotechnology Research Center, Institut Teknologi Bandung

关键词: A genome;    B genome;    β-carotene;    CCD;    D27;   

DOI  :  10.47836/pjtas.44.2.10

学科分类：农业科学（综合）

来源: Universiti Putra Malaysia, Universiti Pertanian Malaysia Press

【 摘 要 】

β-carotene is an important nutritional content in banana. However, its lifetime depends on the enzymes controlling its conversion into strigolactone. To understand the involved enzymes’ activity, which are β-carotene isomerase (D27), carotenoid cleavage dioxygenase 7 (CCD7), and CCD8, would be the key to manipulate the rate of β-carotene degradation. In this research, the structure of genes and proteins of the D27, CCD7, and CCD8 from Musa acuminata were characterized. ‘DH-Pahang’ and Musa balbisiana ‘Pisang Klutuk Wulung’ (PKW). The corresponding sequence of genes from both species were aligned to determine similarity and intron/exon positions. Domains and motifs in the sequences of putative proteins of D27, CCD7, and CCD8 were also identified. It was found that D27, CCD7, and CCD8 genes in DH-Pahang and PKW comprise of various nucleotide sequence length, putative proteins, and numbers and length of exons and introns. However, the putative proteins possess the same domains: DUF4033 (domain of unknown function) in D27 and RPE65 (retinal pigment epithelium) in CCD7 and CCD8. Phylogenetic trees showed that D27, CCD7, and CCD8 proteins from DH-Pahang and PKW are conserved and clustered in the same clades with the same proteins of monocot plants. Hence, the results could be useful for future research in optimizing β-carotene content in banana.

【 授权许可】

Unknown