Expression pattern of olfactory receptor genes in human cumulus cells as an indicator for competent oocyte selection

Turkish journal of biology

article

NEDA DAEI-FARSHBAF¹  REZA AFLATOONIAN²  FATEMEH-SADAT AMJADI¹  SARA TALEAHMAD⁴  MAHNAZ ASHRAFI⁵  MEHRDAD BAKHTIYARI¹ 

[1] Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences;Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, Academic Center for Education, Culture and Research;Cellular and Molecular Research Center, Faculty of Medicine, Iran University of Medical Sciences;Department of Molecular Systems Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology (RI-SCBT), Academic Center for Education, Culture and Research;Department of Obstetrics and Gynecology, Faculty of Medicine, Iran University of Medical Sciences

关键词: Olfactory receptors;    odorant receptors;    G-protein coupled receptors;    adenylyl cyclase type 3;    olfactory marker protein;   

DOI  :  10.3906/biy-2003-79

学科分类：生物科学（综合）

来源: Scientific and Technical Research Council of Turkey - TUBITAK

【 摘 要 】

Odorant or olfactory receptors are mainly localized in the olfactory epithelium for the perception of different odors. Interestingly, many ectopic olfactory receptors with low expression levels have recently been found in nonolfactory tissues to involve in local functions. Therefore, we investigated the probable role of the olfactory signaling pathway in the surrounding microenvironment of oocyte. This study included 22 women in intracytoplasmic sperm injection cycle. The expression of olfactory target molecules in cumulus cells surrounding the growing and mature oocytes was evaluated by Western blotting and real-time polymerase chain reaction. Additionally, integrated bioinformatics analyses were carried out and 6 ectopic olfactory receptors were selected for further evaluation. The initiation of olfactory transduction cascade in cumulus cells of competent oocytes was confirmed by analyzing the expression of adenylyl cyclase type 3 and olfactory market protein. Moreover, the expression pattern of the selected olfactory receptors was evaluated and OR10H2 was selected due to a high level of expression in mature fertile oocytes. We suggested that OR10H2 could be considered as a reliable biomarker for oocyte selection in assisted reproduction technique programs. However, further studies are required to elucidate the role of olfactory transduction cascade in embryo quality and implantation.

【 授权许可】

Unknown