In vitro tooth-shaped scaffold construction by mimicking late bell stage

Turkish journal of biology

article

PAKİZE NESLİHAN TAŞLI¹  GÜL MERVE YALÇIN ÜLKER²  ALEV CUMBUL³  ÜNAL USLU³  ŞAHİN YILMAZ¹  BATUHAN TURHAN BOZKURT¹  FİKRETTİN ŞAHİN¹ 

[1] Department of Genetic and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University;Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, İstanbul Okan University;Department of Histology and Embryology, Faculty of Medicine, Yeditepe University

关键词: Hydroxyapatite;    stem cell;    mesenchymal stem cell;    differentiation;    tissue engineering;    hard tissue engineering;    tooth scaffolding;   

DOI  :  10.3906/biy-2002-19

学科分类：生物科学（综合）

来源: Scientific and Technical Research Council of Turkey - TUBITAK

【 摘 要 】

Neogenesis of osseous and ligamentous interfacial structures is essential for the regeneration of large oral or craniofacial defects. However, current treatment strategies are inadequate in renewing supporting tissues of teeth after trauma, chronic infections or surgical resection. Combined use of 3D scaffolds with stem cells became a promising treatment option for these injuries. Matching different scaffolding materials with different tissues can induce the correct cytokines and the differentiation of cells corresponding to that particular tissue. In this study, a hydroxyapatite (HA) based scaffold was used together with human adipose stem cells (hASCs), human bone marrow stem cells (hBMSCs) and gingival epithelial cells to mimic human tooth dentin-pulp-enamel tissue complexes and model an immature tooth at the late bell stage in vitro. Characteristics of the scaffold were determined via SEM, FTIR, pore size and density measurements. Changes in gene expression, protein secretions and tissue histology resulting from cross-interactions of different dental tissues grown in the system were shown. Classical tooth tissues such as cementum, pulp and bone like tissues were formed within the scaffold. Our study suggests that a HA-based scaffold with different cell lineages can successfully mimic early stages of tooth development and can be a valuable tool for hard tissue engineering.

【 授权许可】

Unknown