Crosstalk between autophagy and apoptosis induced by camphor in Schizosaccharomyces pombe

Turkish journal of biology

article

HIZLAN HINCAL AĞU޹  SEDANUR YILMAZ¹  CANSIN OGEDAY ŞENGÖZ¹ 

[1] Department of Molecular Biology and Genetics, Faculty of Arts & Science, İstanbul Yeni Yüzyıl University

关键词: Camphor;    autophagy;    apoptosis;    Atg8;    Aif1;    Schizosaccharomyces pombe;   

DOI  :  10.3906/biy-1908-11

学科分类：生物科学（综合）

来源: Scientific and Technical Research Council of Turkey - TUBITAK

【 摘 要 】

Camphor is widely used in pharmacy, the food industry, and cosmetics. In this study, we evaluate inhibitory and cytotoxic effects of camphor in the fission yeast ( Schizosaccharomyces pombe ), which presents a unicellular model in mechanistic toxicology and cell biology. Low-dose camphor exposure (0.4 mg/mL) activated autophagy, which was shown by GFP-Atg8 dots and transcriptional upregulation of Atg6 (Beclin-1 ortholog). Autophagy was also confirmed by using autophagy-deficient cells, which showed reduction in GFP-Atg8 dot formation. However, high-dose camphor exposure (0.8 mg/mL) caused dramatic cell death ratios, demonstrated by spot and colony-forming assays, even in autophagy-deficient cells. To unravel the underlying mechanism, this time, apoptosis-deficient cells were exposed to low- and high-dose camphor. Apoptosis was also confirmed by acridine orange/ethidium bromide staining. Among yeast apoptosis mediators, Aif1 was found to mediate camphor-induced cell death. In conclusion, differential regulation of autophagy and apoptosis, and switches between them, were found to be dose-dependent. The potential effects of camphor on autophagy and apoptotic cell death and underlying mechanisms were clarified in basic unicellular eukaryotic model, S. pombe .

【 授权许可】

Unknown