Multispectral intravital microscopy for simultaneous bright-field and fluorescence imaging of the microvasculature

Applied Microscopy

Jeffrey J. L. Carson¹  Barry G. H. Janssen²  Mohamadreza Najiminaini³  Parsa Omidi⁴  Yan Min Zhang⁵ 

[1]Department of Medical Biophysics, Western University, N6A 5C1, London, ON, Canada

[2]Imaging Program, St.Joseph’s Health Care, Lawson Health Research Institute, N6A 4V2, London, ON, Canada

[3]Department of Pathology, Western University, N6A 5C1, London, ON, Canada

[4]Intensive Care Unit, Tianjin Nankai Hospital, 300100, Tianjin, People’s Republic of China

[5]Department of Medical Biophysics, Western University, N6A 5C1, London, ON, Canada

[6]Kidney Clinical Research Unit (KCRU), London Health Sciences Centre, N6C 6B5, London, ON, Canada

[7]Imaging Program, St.Joseph’s Health Care, Lawson Health Research Institute, N6A 4V2, London, ON, Canada

[8]Department of Pathology, Western University, N6A 5C1, London, ON, Canada

[9]Imaging Program, St.Joseph’s Health Care, Lawson Health Research Institute, N6A 4V2, London, ON, Canada

[10]Department of Pathology, Western University, N6A 5C1, London, ON, Canada

[11]Intensive Care Unit, Tianjin Nankai Hospital, 300100, Tianjin, People’s Republic of China

[12]Kidney Clinical Research Unit (KCRU), London Health Sciences Centre, N6C 6B5, London, ON, Canada

[13]Trauma Research Centre, Fourth Medical Center of the Chinese PLA General Hospital, 100048, Beijing, People’s Republic of China

[14]School of Biomedical Engineering, Western University, N6A 3K7, London, ON, Canada

[15]Intensive Care Unit, Tianjin Nankai Hospital, 300100, Tianjin, People’s Republic of China

关键词: Multispectral microscopy;    Brightfield microscopy;    Fluorescence microscopy;    Intravital microscopy;   

DOI  :  10.1186/s42649-021-00059-6

来源: Springer

【 摘 要 】

Intravital video microscopy permits the observation of microcirculatory blood flow. This often requires fluorescent probes to visualize structures and dynamic processes that cannot be observed with conventional bright-field microscopy. Conventional light microscopes do not allow for simultaneous bright-field and fluorescent imaging. Moreover, in conventional microscopes, only one type of fluorescent label can be observed. This study introduces multispectral intravital video microscopy, which combines bright-field and fluorescence microscopy in a standard light microscope. The technique enables simultaneous real-time observation of fluorescently-labeled structures in relation to their direct physical surroundings. The advancement provides context for the orientation, movement, and function of labeled structures in the microcirculation.

【 授权许可】

CC BY