Dynamic changes of miRNAs in skeletal muscle development at New Zealand rabbits

BMC Genomics

Yixiao Zhu¹  Jiao Wang¹  Yinghui Ling¹  Qi Zheng¹  Shuaiqi Qin¹  Jing Jing¹  Jingtong Huang¹  Qianyun Ji¹  Cuiyun Zhu¹  Xichun Jiang²  Huiqun Yin³ 

[1] College of Animal Science and Technology, Anhui Agricultural University, 230036, Hefei, Anhui, People’s Republic of China;Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio- Breeding, Anhui Agricultural University, 230036, Hefei, Anhui, People’s Republic of China;Institute of Animal Science and Veterinary Medicine, Anhui Academy of Agricultural Sciences, 230031, Hefei, Anhui, People’s Republic of China;Reproductive Medicine Center, The 901st Hospital, 230031, Hefei, Anhui, People’s Republic of China;

关键词: miRNA;    Skeletal muscle;    sRNA-seq;    Rabbit;    Development;   

DOI  :  10.1186/s12864-021-07896-5

来源: Springer

【 摘 要 】

BackgroundmiRNA is one of the crucial roles in the complex and dynamic network that regulates the development of skeletal muscle. The landscape of skeletal muscle miRNAs from fetus to adult in New Zealand rabbits has not been revealed yet.ResultsIn this study, nine RNA-seq libraries of fetus, child and adult rabbits’ leg muscles were constructed. A total of 278 differentially expressed miRNAs (DEmiRNAs) were identified. In the fetus vs. child group, the main functional enrichments were involved in membrane and transport. Pathway enriched terms of up-regulated DEmiRNAs were connected with the differentiation and hypertrophy of skeletal muscle, and down-regulated ones were related to muscle structure and metabolic capacity. In the child vs. adult group, functions were associated to positioning and transportation, and pathways were relevant to ECM, muscle structure and hypertrophy. Finally, ocu-miR-185-3p and ocu-miR-370-3p, which had the most target genes, were identified as hub-miRNAs in these two groups.ConclusionsIn short, we summarized the highly expressed and uniquely expressed DEmiRNAs of fetus, child and adult rabbits’ leg muscles. Besides, the potential functional changes of miRNAs in two consecutive stages have been explored. Among them, the ocu-miR-185-3p and ocu-miR-370-3p with the most target genes were selected as hub-miRNAs. These data improved the understanding of the regulatory molecules of meat rabbit development, and provided a novel perspective for molecular breeding of meat rabbits.

【 授权许可】

CC BY