Synthesis of ellagic acid glucoside using glucansucrase from Leuconostoc and characterization of this glucoside as a functional neuroprotective agent

AMB Express

Kwang-Yeol Yang¹  Jeong-Yong Cho²  Hana Jeong³  Hyejin Yu³  Seung-Hee Nam⁴  In Ki Hong⁵ 

[1] Department of Applied Biology Graduate School, Chonnam National University, 61186, Gwangju, South Korea;Division of Food Technology & Biotechnology, Chonnam National University, 61186, Gwangju, South Korea;Institute of Agricultural Science and Technology, Chonnam National University, 61186, Gwangju, South Korea;Institute of Agricultural Science and Technology, Chonnam National University, 61186, Gwangju, South Korea;Division of Food Technology & Biotechnology, Chonnam National University, 61186, Gwangju, South Korea;Kolmar Korea R&D Center, 61, Heolleung-ro 8-gil, Seocho-gu, 06800, Seoul, Republic of Korea;

关键词: Ellagic acid;    Glucansucrase;    Leuconostoc mesenteroides;    Transglucosylation;    Brain protective effect;   

DOI  :  10.1186/s13568-021-01265-x

来源: Springer

【 摘 要 】

Ellagic acid glucoside was synthesized via transglucosylation using sucrose and glucansucrase derived from Leuconostoc mesenteroides B-512 FMCM. After such enzymatic synthesis, the product was purified by 50% ethyl acetate fraction and C18 column chromatography. Modification of ellagic acid glucoside was verified by LC–MS/MS at m/z 485.1 (M + Na)− and m/z 531.1 (M + 3Na)−. The yield of ellagic acid glucoside was 69% (3.47 mM) by response surface methodology using 150 mM sucrose, 300 mU/mL glucansucrase, and 5 mM ellagic acid. The synthesized ellagic acid glucoside showed improved water solubility, up to 58% higher brain nerve cell (SH-SY5Y) protective effect, threefold higher cortisol reducing effect, and fourfold stronger inhibitory effect on acetylcholinesterase (AChE) than ellagic acid. These results indicate that ellagic acid glucoside could be used as a neuroprotective agent.

【 授权许可】

CC BY