AMB Express | |
Synthesis of ellagic acid glucoside using glucansucrase from Leuconostoc and characterization of this glucoside as a functional neuroprotective agent | |
Kwang-Yeol Yang1  Jeong-Yong Cho2  Hana Jeong3  Hyejin Yu3  Seung-Hee Nam4  In Ki Hong5  | |
[1] Department of Applied Biology Graduate School, Chonnam National University, 61186, Gwangju, South Korea;Division of Food Technology & Biotechnology, Chonnam National University, 61186, Gwangju, South Korea;Institute of Agricultural Science and Technology, Chonnam National University, 61186, Gwangju, South Korea;Institute of Agricultural Science and Technology, Chonnam National University, 61186, Gwangju, South Korea;Division of Food Technology & Biotechnology, Chonnam National University, 61186, Gwangju, South Korea;Kolmar Korea R&D Center, 61, Heolleung-ro 8-gil, Seocho-gu, 06800, Seoul, Republic of Korea; | |
关键词: Ellagic acid; Glucansucrase; Leuconostoc mesenteroides; Transglucosylation; Brain protective effect; | |
DOI : 10.1186/s13568-021-01265-x | |
来源: Springer | |
【 摘 要 】
Ellagic acid glucoside was synthesized via transglucosylation using sucrose and glucansucrase derived from Leuconostoc mesenteroides B-512 FMCM. After such enzymatic synthesis, the product was purified by 50% ethyl acetate fraction and C18 column chromatography. Modification of ellagic acid glucoside was verified by LC–MS/MS at m/z 485.1 (M + Na)− and m/z 531.1 (M + 3Na)−. The yield of ellagic acid glucoside was 69% (3.47 mM) by response surface methodology using 150 mM sucrose, 300 mU/mL glucansucrase, and 5 mM ellagic acid. The synthesized ellagic acid glucoside showed improved water solubility, up to 58% higher brain nerve cell (SH-SY5Y) protective effect, threefold higher cortisol reducing effect, and fourfold stronger inhibitory effect on acetylcholinesterase (AChE) than ellagic acid. These results indicate that ellagic acid glucoside could be used as a neuroprotective agent.
【 授权许可】
CC BY
【 预 览 】
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