In vivo evaluation of a recombinant N-acylhomoserine lactonase formulated in a hydrogel using a murine model infected with MDR Pseudomonas aeruginosa clinical isolate, CCASUP2

AMB Express

Khaled M. Aboshanab¹  Mahmoud A. Yassien¹  Masarra M. Sakr¹  Nadia A. Hassouna¹  Walid F. Elkhatib²  Eman M. Mantawy³ 

[1] Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, 11566, African Union Organization St. Abbassia, Cairo, Egypt;Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, 11566, African Union Organization St. Abbassia, Cairo, Egypt;Department of Microbiology & Immunology, Faculty of Pharmacy, Galala University, New Galala city, Suez, Egypt;Department of Pharmacology and Toxicology, Faculty of Pharmacy, Ain Shams University, 11566, African Union Organization St. Abbassia, Cairo, Egypt;

关键词: Quorum quenching;    N-acylhomoserine lactonase;    P. aeruginosa;    Burn infection;   

DOI  :  10.1186/s13568-021-01269-7

来源: Springer

【 摘 要 】

Failure in the treatment of P. aeruginosa, due to its broad spectrum of resistance, has been associated with increased patient mortality. One alternative approach for infection control is quorum quenching which was found to decrease virulence of such pathogen. In this study, the efficiency of a recombinant Ahl-1 lactonase formulated as a hydrogel was investigated to control the infection of multidrug resistant (MDR) P. aeruginosa infected burn using a murine model. The recombinant N-acylhomoserine lactonase (Ahl-1) was formulated as a hydrogel. To test its ability to control the infection of MDR P. aeruginosa, a thermal injury model was used. Survival rate, and systemic spread of the infection were evaluated. Histopathological examination of the animal dorsal skin was also done for monitoring the healing and cellular changes at the site of infection. Survival rate in the treated group was 100% relative to 40% in the control group. A decrease of up to 3 logs of bacterial count in the blood samples of the treated animals relative to the control group and a decrease of up to 4 logs and 2.3 logs of bacteria in lung and liver samples, respectively were observed. Histopathological examination revealed more enhanced healing process in the treated group. Accordingly, by promoting healing of infected MDR P. aeruginosa burn and by reducing systemic spread of the infection as well as decreasing mortality rate, Ahl-1 hydrogel application is a promising strategy that can be used to combat and control P. aeruginosa burn infections.

【 授权许可】

CC BY