Serological and molecular epidemiology of the Dengue, Zika and Chikungunya viruses in a risk area in Brazil

BMC Infectious Diseases

Maria do Socorro Lucena¹  Glaubervânio Leite Tavares Pereira²  João Marcos Ferreira de Lima Silva²  Anete Grumach³  Magaly Lima Mota⁴  Shirley Vasconcelos Komninakis⁵  Ester Cerdeira Sabino⁶  James Hunter⁷  Robson dos Santos Souza Marinho⁷  Rodrigo Lopes Sanz Duro⁷  Ricardo Sobhie Diaz⁷  Irwin Rose Alencar de Menezes⁸ 

[1] Central Public Health Laboratory, 63010-464, Juazeiro do Norte, CE, Brazil;Centro Universitário Dr. Leão Sampaio, 63040-405, Juazeiro do Norte, CE, Brazil;Faculty of Medical of the ABC, 09060-870, Santo André, SP, Brazil;Faculty of Medical of the ABC, 09060-870, Santo André, SP, Brazil;Centro Universitário Dr. Leão Sampaio, 63040-405, Juazeiro do Norte, CE, Brazil;Faculty of Medical of the ABC, 09060-870, Santo André, SP, Brazil;Retrovirology Laboratory, Federal University of São Paulo, 04039-032, São Paulo, SP, Brazil;Institute of Tropical Medicine, University of São Paulo, 05403-000, São Paulo, SP, Brazil;Retrovirology Laboratory, Federal University of São Paulo, 04039-032, São Paulo, SP, Brazil;Universidade Regional do Cariri, 63105-010, Crato, CE, Brazil;

关键词: Epidemiology;    Dengue;    Zika;    Chikungunya;    Brazil;   

DOI  :  10.1186/s12879-021-06401-3

来源: Springer

【 摘 要 】

BackgroundThe co-circulation of types of arbovirus in areas where they are endemic increased the risk of outbreaks and limited the diagnostic methods available. Here, we analyze the epidemiological profile of DENV, CHIKV and ZIKV at the serological and molecular level in patients with suspected infection with these arboviruses in the city of Juazeiro do Norte, Ceará, Brazil.MethodsIn 2016, the Central Public Health Laboratory (LACEN) of Juazeiro do Norte received 182 plasma samples from patients who visited health facilities with symptoms compatible with arbovirus infection. The LACEN performed serological tests for detection of IgM/IgG to DENV and CHIKV. They then sent these samples to the Retrovirology Laboratory of the Federal University of São Paulo and Faculty of Medical of the ABC where molecular analyses to confirm the infection by DENV, ZIKV and CHIKV were performed. The prevalence of IgM/IgG antibodies and of infections confirmed by RT-qPCR were presented with 95% confidence interval.ResultsIn serologic analysis, 125 samples were positive for antibodies against CHIKV and all were positive for antibodies against DENV. A higher prevalence of IgG against CHIKV (63.20% with 95% CI: 45.76–70.56) than against DENV (95.05% with 95% CI: 78.09–98.12) was observed. When the samples were submitted to analysis by RT-qPCR, we observed the following prevalence: mono-infection by ZIKV of 19.23% (95% CI: 14.29–34.82) patients, mono-infection by CHIKV of 3.84% (95% CI: 2.01–5.44) and co-infection with ZIKV and CHIKV of 1.09% (95% CI: 0.89–4.56).ConclusionThe serologic and molecular tests performed in this study were effective in analyzing the epidemiological profile of DENV, CHIKV and ZIKV in patients with suspected infection by these arboviruses in the city of Juazeiro do Norte, Ceará/Brazil.

【 授权许可】

CC BY