Mining methods and typical structural mechanisms of terpene cyclases

Bioresources and Bioprocessing

Ai-Tao Li¹  Zheng-Yu Huang²  Jian-He Xu²  Ru-Yi Ye²  Hui-Lei Yu² 

[1] State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, 430062, Wuhan, China;State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Centre for Biomanufacturing, Frontiers Science Center for Materiobiology and Dynamic Chemistry, East China University of Science and Technology, 200237, Shanghai, China;

关键词: Terpene cyclases;    Biochemical properties;    Review;    Genomic mining;    Structural analysis;    Catalytic mechanisms;   

DOI  :  10.1186/s40643-021-00421-2

来源: Springer

【 摘 要 】

Terpenoids, formed by cyclization and/or permutation of isoprenes, are the most diverse and abundant class of natural products with a broad range of significant functions. One family of the critical enzymes involved in terpenoid biosynthesis is terpene cyclases (TCs), also known as terpene synthases (TSs), which are responsible for forming the ring structure as a backbone of functionally diverse terpenoids. With the recent advances in biotechnology, the researches on terpene cyclases have gradually shifted from the genomic mining of novel enzyme resources to the analysis of their structures and mechanisms. In this review, we summarize both the new methods for genomic mining and the structural mechanisms of some typical terpene cyclases, which are helpful for the discovery, engineering and application of more and new TCs.

【 授权许可】

CC BY