Assessing the antigenicity of different VP3 regions of infectious bursal disease virus in chickens from South Brazil

BMC Veterinary Research

Ana Paula Gori Palka¹  Stenio Perdigão Fragoso²  Tatiana Reichert Assunção de Matos²  Danilo Santos Eugênio³  Claudemir de Souza³  Daniela Parada Pavoni⁴  Marco Aurélio Krieger⁴ 

[1] Instituto Carlos Chagas/ICC, Fundação Oswaldo Cruz/Fiocruz, Curitiba, PR, Brazil;Instituto de Tecnologia do Paraná/Tecpar, Curitiba, PR, Brazil;Programa de Pós-graduação em Biologia Celular e Molecular, Universidade Federal do Paraná/UFPR, Curitiba, PR, Brazil;Instituto Carlos Chagas/ICC, Fundação Oswaldo Cruz/Fiocruz, Curitiba, PR, Brazil;Programa de Pós-graduação em Biociências e Biotecnologia, Instituto Carlos Chagas/ICC, Fundação Oswaldo Cruz/Fiocruz, Curitiba, PR, Brazil;Instituto Carlos Chagas/ICC, Fundação Oswaldo Cruz/Fiocruz, Curitiba, PR, Brazil;Programa de Pós-graduação em Biologia Celular e Molecular, Universidade Federal do Paraná/UFPR, Curitiba, PR, Brazil;Instituto Carlos Chagas/ICC, Fundação Oswaldo Cruz/Fiocruz, Curitiba, PR, Brazil;Programa de Pós-graduação em Biologia Celular e Molecular, Universidade Federal do Paraná/UFPR, Curitiba, PR, Brazil;Programa de Pós-graduação em Biociências e Biotecnologia, Instituto Carlos Chagas/ICC, Fundação Oswaldo Cruz/Fiocruz, Curitiba, PR, Brazil;

关键词: Infectious bursal disease;    Gumboro disease;    VP3;    Antigenic regions;    ELISA;   

DOI  :  10.1186/s12917-021-02956-0

来源: Springer

【 摘 要 】

BackgroundInfectious bursal disease (IBD), also known as Gumboro disease, is a viral infection that causes mortality and immunosuppression in chickens (Gallus gallus). VP2 and VP3 are the major structural viral capsid components and are the most immunogenic proteins of IBD virus (IBDV). Reliable diagnostic tests using VP2 and VP3 produced in heterologous systems are important tools to control this infection. One advantage of an IBD diagnostic based on VP3, over those that use VP2, is that VP3 has linear epitopes, enabling its production in bacteria.ResultsWe tested the suitability of recombinant VP3 (rVP3) as a diagnostic reagent in an enzyme-linked immunosorbent assay (ELISA). Compared with a commercial test, rVP3 ELISA showed high sensitivity and specificity as a diagnostic tool for vaccinated animals. In addition, rVP3, but not the commercial ELISA, was able to detect antibodies in nonvaccinated chickens, probably developed against circulating IBDV strains. It was possible the assessment of VP3 regions antigenicity using chicken antisera.ConclusionsThe full-length recombinant VP3 can be used to assess post vaccination immunological status of chickens and its production is feasible and inexpensive. The evaluation of VP3 regions as candidates for general use in the diagnosis of IBD in chickens should be conducted with caution. Our work was the first to identify several regions of VP3 recognized by chicken antibodies.

【 授权许可】

CC BY