Electric signals counterbalanced posterior vs anterior PTEN signaling in directed migration of Dictyostelium

Cell & Bioscience

Christopher Janetopoulos¹  Zhipeng Liu²  Tao Yin²  Min Zhao³  Yu Gu⁴  Wayne Nishio Ayre⁴  Ying Li⁵  Bing Song⁶  Wenkai Jiang⁶  Miho Iijima⁷  Peter Devreotes⁷ 

[1] BioImaging Core Facility, University of Sciences, 19104, Philadelphia, PA, USA;Chinese Academy of Medical Sciences & Peking Union Medical College Institute of Biomedical Engineering, Tianjin, China;Department of Ophthalmology & Vision Science, UC Davis, School of Medicine, 95618, Davis, CA, USA;Department of Dermatology, UC Davis, School of Medicine, 95618, Davis, CA, USA;School of Dentistry, College of Biomedical and Life Sciences, Cardiff University, CF14 4XY, Cardiff, UK;School of Dentistry, College of Biomedical and Life Sciences, Cardiff University, CF14 4XY, Cardiff, UK;Chinese Academy of Medical Sciences & Peking Union Medical College Institute of Biomedical Engineering, Tianjin, China;School of Dentistry, College of Biomedical and Life Sciences, Cardiff University, CF14 4XY, Cardiff, UK;State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases, School of Stomatology, Fourth Military Medical University, Xi’an, China;School of Medicine, Johns Hopkins University, 21205, Baltimore, MD, USA;

关键词: PTEN;    PIP3;    Myosin;    PH-Crac;    F-actin;    Electric field;    Cell migration;    Electrotaxis;    Galvanotaxis;   

DOI  :  10.1186/s13578-021-00580-x

来源: Springer

【 摘 要 】

BackgroundCells show directed migration response to electric signals, namely electrotaxis or galvanotaxis. PI3K and PTEN jointly play counterbalancing roles in this event via a bilateral regulation of PIP3 signaling. PI3K has been proved essential in anterior signaling of electrotaxing cells, whilst the role of PTEN remains elusive.MethodsDictyostelium cells with different genetic backgrounds were treated with direct current electric signals to investigate the genetic regulation of electrotaxis.ResultsWe demonstrated that electric signals promoted PTEN phosphatase activity and asymmetrical translocation to the posterior plasma membrane of the electrotaxing cells. Electric stimulation produced a similar but delayed rear redistribution of myosin II, immediately before electrotaxis started. Actin polymerization is required for the asymmetric membrane translocation of PTEN and myosin. PTEN signaling is also responsible for the asymmetric anterior redistribution of PIP3/F-actin, and a biased redistribution of pseudopod protrusion in the forwarding direction of electrotaxing cells.ConclusionsPTEN controls electrotaxis by coordinately regulating asymmetric redistribution of myosin to the posterior, and PIP3/F-actin to the anterior region of the directed migration cells.

【 授权许可】

CC BY