SARS-CoV-2 PCR and antibody testing for an entire rural community: methods and feasibility of high-throughput testing procedures

Archives of Public Health

Doug MacIntosh¹  Katherine Nicholson¹  Jonathan Kramer-Feldman²  Gabriel Chamie²  Jonathan Im²  Bryan Greenhouse²  Diane Havlir²  Joanna Vinden²  Shahryar Rahdari²  Aenor Sawyer²  Kimberly Baltzell²  Salu Ribeiro²  Kathryn Dippell²  Elias Duarte²  Ayesha Appa²  CLIAHUB Consortium³ 

[1] San Francisco State University, San Francisco, USA;University of California, San Francisco Division of HIV, Infectious Diseases, and Global Medicine, San Francisco, CA, USA;University of California, San Francisco Division of HIV, Infectious Diseases, and Global Medicine, San Francisco, CA, USA;Chan Zuckerberg Biohub, San Francisco, USA;

关键词: COVID-19;    COVID-19 diagnostic testing;    Rural Population;    Feasability Studies;   

DOI  :  10.1186/s13690-021-00647-8

来源: Springer

【 摘 要 】

BackgroundEarly in the pandemic, inadequate SARS-CoV-2 testing limited understanding of transmission. Chief among barriers to large-scale testing was unknown feasibility, particularly in non-urban areas. Our objective was to report methods of high-volume, comprehensive SARS-CoV-2 testing, offering one model to augment disease surveillance in a rural community.MethodsA community-university partnership created an operational site used to test most residents of Bolinas, California regardless of symptoms in 4 days (April 20th – April 23rd, 2020). Prior to testing, key preparatory elements included community mobilization, pre-registration, volunteer recruitment, and data management. On day of testing, participants were directed to a testing lane after site entry. An administrator viewed the lane-specific queue and pre-prepared test kits, linked to participants’ records. Medical personnel performed sample collection, which included finger prick with blood collection to run laboratory-based antibody testing and respiratory specimen collection for polymerase chain reaction (PCR).ResultsUsing this 4-lane model, 1,840 participants were tested in 4 days. A median of 57 participants (IQR 47–67) were tested hourly. The fewest participants were tested on day 1 (n = 338 participants), an intentionally lower volume day, increasing to n = 571 participants on day 4. The number of testing teams was also increased to two per lane to allow simultaneous testing of multiple participants on days 2–4. Consistent staffing on all days helped optimize proficiency, and strong community partnership was essential from planning through execution.ConclusionsHigh-volume ascertainment of SARS-CoV-2 prevalence by PCR and antibody testing was feasible when conducted in a community-led, drive-through model in a non-urban area.

【 授权许可】

CC BY