A novel large animal model of smoke inhalation-induced acute respiratory distress syndrome

Respiratory Research

Roser Romaguera Matas¹  Andrea McCain¹  Ernesto Martinez Duarte²  Keely L. Buesing³  Premila D. Leiphrakpam³  Hannah R. Weber³ 

[1] Department of Mechanical and Materials Engineering, University of Nebraska-Lincoln, Lincoln, NE, USA;Department of Pathology and Microbiology, College of Medicine, University of Nebraska Medical Center, Omaha, NE, USA;Department of Surgery, College of Medicine, University of Nebraska Medical Center, 68198-3280, Omaha, NE, USA;

关键词: Smoke inhalation;    ARDS;    Large animal model;    Toxic inhalation injury;    Hypoxia;    Hypoxemia;   

DOI  :  10.1186/s12931-021-01788-8

来源: Springer

【 摘 要 】

BackgroundAcute respiratory distress syndrome (ARDS) is multifactorial and can result from sepsis, trauma, or pneumonia, amongst other primary pathologies. It is one of the major causes of death in critically ill patients with a reported mortality rate up to 45%. The present study focuses on the development of a large animal model of smoke inhalation-induced ARDS in an effort to provide the scientific community with a reliable, reproducible large animal model of isolated toxic inhalation injury-induced ARDS.MethodsAnimals (n = 21) were exposed to smoke under general anesthesia for 1 to 2 h (median smoke exposure = 0.5 to 1 L of oak wood smoke) after the ultrasound-guided placement of carotid, pulmonary, and femoral artery catheters. Peripheral oxygen saturation (SpO2), vital signs, and ventilator parameters were monitored throughout the procedure. Chest x-ray, carotid, femoral and pulmonary artery blood samples were collected before, during, and after smoke exposure. Animals were euthanized and lung tissue collected for analysis 48 h after smoke inhalation.ResultsAnimals developed ARDS 48 h after smoke inhalation as reflected by a decrease in SpO2 by approximately 31%, PaO2/FiO2 ratio by approximately 208 (50%), and development of bilateral, diffuse infiltrates on chest x-ray. Study animals also demonstrated a significant increase in IL-6 level, lung tissue injury score and wet/dry ratio, as well as changes in other arterial blood gas (ABG) parameters.ConclusionsThis study reports, for the first time, a novel large animal model of isolated smoke inhalation-induced ARDS without confounding variables such as cutaneous burn injury. Use of this unique model may be of benefit in studying the pathophysiology of inhalation injury or for development of novel therapeutics.

【 授权许可】

CC BY