Genome-wide identification and characterization of caffeoyl-coenzyme A O-methyltransferase genes related to the Fusarium head blight response in wheat

BMC Genomics

Qifan Guo¹  Weijun Zheng¹  Guang Yang¹  Yan Pan¹  Wenqiu Pan¹  Ruoyu Zhang¹  Weining Song²  Xiaojun Nie² 

[1]State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy and Yangling Branch of China Wheat Improvement Center, Northwest A&F University, 712100, Yangling, Shaanxi, China

[2]State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy and Yangling Branch of China Wheat Improvement Center, Northwest A&F University, 712100, Yangling, Shaanxi, China

[3]ICARDA-NWSUAF Joint Research Centre, Northwest A&F University, 712100, Yangling, Shaanxi, China

关键词: CCoAOMT gene family;    Fusarium head blight (FHB);    Expression pattern;    Regulation module;    Genetic variation;    Wheat;   

DOI  :  10.1186/s12864-021-07849-y

来源: Springer

【 摘 要 】

BackgroundLignin is one of the main components of the cell wall and is directly associated with plant development and defence mechanisms in plants, especially in response to Fusarium graminearum (Fg) infection. Caffeoyl-coenzyme A O-methyltransferase (CCoAOMT) is the main regulator determining the efficiency of lignin synthesis and composition. Although it has been characterized in many plants, to date, the importance of the CCoAOMT family in wheat is not well understood.ResultsHere, a total of 21 wheat CCoAOMT genes (TaCCoAOMT) were identified through an in silico genome search method and they were classified into four groups based on phylogenetic analysis, with the members of the same group sharing similar gene structures and conserved motif compositions. Furthermore, the expression patterns and co-expression network in which TaCCoAOMT is involved were comprehensively investigated using 48 RNA-seq samples from Fg infected and mock samples of 4 wheat genotypes. Combined with qRT-PCR validation of 11 Fg-responsive TaCCoAOMT genes, potential candidates involved in the FHB response and their regulation modules were preliminarily suggested. Additionally, we investigated the genetic diversity and main haplotypes of these CCoAOMT genes in bread wheat and its relative populations based on resequencing data.ConclusionsThis study identified and characterized the CCoAOMT family in wheat, which not only provided potential targets for further functional analysis, but also contributed to uncovering the mechanism of lignin biosynthesis and its role in FHB tolerance in wheat and beyond.

【 授权许可】

CC BY