The Bdkrb2 gene family provides a novel view of viviparity adaptation in Sebastes schlegelii

BMC Evolutionary Biology

article

Niu, Jingjing¹  Song, Weihao¹  Li, Rui¹  Yu, Haiyang¹  Guan, Jian¹  Qi, Jie¹  He, Yan¹ 

[1] MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China

关键词: Bdkrb2;    Viviparity;    Ovarian wall;    Vasodilatation;    Adaptation;   

DOI  :  10.1186/s12862-021-01774-0

学科分类：护理学

来源: BioMed Central

【 摘 要 】

Black rockfish (Sebastes schlegelii) is a viviparous teleost. We proposed that the rockfish ovarian wall had a similar function to the uterus of mammals previously. In the present study, the well-developed vascular system was observed in the ovarian wall and the exterior surface of the egg membrane. In gestation, adaptation of the ovary vasculature to the rising needs of the embryos occurs through both vasodilation and neovascularization. Bdkrb2, encoding a receptor for bradykinin, plays a critical role in the control of vasodilatation by regulating nitric oxide production. Eight Bdkrb2 genes were identified in the black rockfish genome. These genes were located on chromosome 14, which are arranged in a tandem array, forming a gene cluster spanning 50 kb. Protein structure prediction, phylogenetic analysis, and transcriptome analysis showed that eight Bdkrb2 genes evolved two kinds of protein structure and three types of tissue expression pattern. Overexpression of two Bdkrb2 genes in zebrafish indicated a role of them in blood vessel formation or remodeling, which is an important procedure for the viviparous rockfish getting prepared for fertilization and embryos implantation. Our study characterizes eight Bdrkb2 genes in the black rockfish, which may contribute to preparation for fertilization and embryo implantation. This research provides a novel view of viviparity adaptation and lays the groundwork for future research into vascular regulation of ovarian tissue in the breeding cycle in black rockfish.

【 授权许可】

CC BY|CC0