Visualization of integrin molecules by fluorescence imaging and techniques

Biocell

article

CHEN CAI¹  HAO SUN²  LIANG HU³  ZHICHAO FAN¹ 

[1] Department of Immunology, School of Medicine, UConn Health, Farmington;Department of Medicine, University of California;Cardiovascular Institute of Zhengzhou University, Department of Cardiology, The First Affiliated Hospital of Zhengzhou University

关键词: Integrins;    Fluorescence imaging;    Fluorescence labeling;    Live-cell imaging;    Super-resolution imaging;    Intravital imaging;    FRET;   

DOI  :  10.32604/biocell.2021.014338

学科分类：仪器

来源: Biocell

【 摘 要 】

Integrin molecules are transmembrane αβ heterodimers involved in cell adhesion, trafficking, and signaling. Upon activation, integrins undergo dynamic conformational changes that regulate their affinity to ligands. The physiological functions and activation mechanisms of integrins have been heavily discussed in previous studies and reviews, but the fluorescence imaging techniques –which are powerful tools for biological studies– have not. Here we review the fluorescence labeling methods, imaging techniques, as well as Förster resonance energy transfer assays used to study integrin expression, localization, activation, and functions.

【 授权许可】

CC BY