Acetyl-L-carnitine protects adipose-derived stem cells exposed to H 2 O 2 through regulating AMBRA1-related autophagy

Biocell

article

YAO QIAN¹  LIQUN LI¹  MING LIN²  JINGPING WANG¹  ZIWAN JI¹  HAO CHEN¹  YUCANG HE¹  XULING LV¹  ZIKAI ZHANG¹  TIAN LI¹  TIANYUN PAN³ 

[1] The First Affiliated Hospital of Wenzhou Medical University;The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University;Huzhou Hospital of Traditional Chinese Medicine

关键词: Oxidative stress;    Cell activity;    Autophagy-related proteins;    Antioxidant enzymes;   

DOI  :  10.32604/biocell.2021.011827

学科分类：仪器

来源: Biocell

【 摘 要 】

The cell activity of adipose-derived stem cells (ADSCs) is affected by the intracellular reactive oxygen species (ROS) and the level of autophagy. Previous studies reveal that acetyl-L-carnitine (ALC) possesses capacities of resisting oxidative stress and regulating autophagy. Activating molecule in Beclin1-regulated autophagy protein 1 (AMBRA1) plays a key role in initiating Beclin1-regulated autophagy. In the present study, we discovered ALC pretreatment (1 mM, 24 h) significantly increased the activity of ADSCs exposed to H 2 O 2 (100 μM, 2 h) in vitro with improved stemness, and reduced the production of intracellular ROS. In addition, we found for the first time that ALC treatment up-regulated autophagy of ADSCs through strengthening the expressions of Beclin1 and AMBRA1 synchronously, which might be involved in the protective effect of ALC.

【 授权许可】

CC BY