Reversal of multidrug resistance and antitumor promoting activity of 3-oxo-6β-hydroxy- β-amyrin isolated from Pistacia integerrima

Biocell

article

ABDUR RAUF¹  JOSEPH MOLNAR²  AKOS CSONKA²  DIÁNA SZABÓ³  HAROON KHAN⁴  MOHAMMAD S. MUBARAK⁵  TAIBI BEN HADDA⁶  MUDYAWATI KAMARUDDIN⁷  SEEMA PATEL⁸  SAUD BAWAZEER⁹  MUSLIM RAZA^1,10  EMAN EL-SHARKAWY^1,11  MD. HABIBUR RAHMAN^1,12  MOHAMED A. EL-ESAWI^1,14  GHIAS UDDIN^1,15  BINA S. SIDDIQUI^1,16  ANEES AHMED KHALIL^1,17 

[1] Department of Chemistry, University of Swabi;Department of Medical Microbiology and Immunobiology, Faculty of Medicine, University of Szeged;Department of Oto-Rhino-Laryngology and Head-Neck Surgery, Faculty of Medicine, University of Szeged;Department of Pharmacy, Abdul Wali Khan University;Department of Chemistry, The University of Jordan;LCM Laboratory, Faculty of Sciences, Mohamed First University;Institution of Health Science of Tahirah Al Baeti Bulukumba;Bioinformatics and Medical Informatics Research Center, San Diego State University;Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Umm Al-Qura University;State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology;Chemical Departments, Faculty of Science, Northern Borders University;Department of Pharmacy, Southeast University;Department of Global Medical Science, Wonju College of Medicine, Yonsei University;Botany Department, Faculty of Science, Tanta University;Institute of Chemical Sciences, University of Peshawar;H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi;University Institute of Diet and Nutritional Sciences, Faculty of Allied Health Sciences, The University of Lahore

关键词: Pistacia integerrima;    Anti-tumor properties;    X-ray crystallography;    POM;    Molecular docking;   

DOI  :  10.32604/biocell.2021.013277

学科分类：仪器

来源: Biocell

【 摘 要 】

The bioactive triterpenoid 3-oxo-6-β-hydroxy-β-amyrin (1) has been isolated from multiple plant sources. In this study, chloroform fraction of Pistacia integerrima extract was processed for the isolation of the compound. The compound identity was confirmed by advanced spectroscopy technique. X-ray crystallography was applied for molecular structure confirmation. In addition, compound 1 was screen for its activity on reversal of MDR (multidrug resistance) mediated by P-gp (P-glycoprotein). This was accomplished by using rhodamine123 exclusion on multidrug-resistant human ABCB1 gene transfected mouse T-lymphoma cell line. Outcomes revealed that MDR reversing effect was comparable to verapamil as positive control in vitro . Treatment of TPA-induced tumor promotion with 3-oxo-6β-hydroxy- β-amyrin led to reduction in the applied anti-tumor promotion experiment. The chemo-preventive effect of 3-oxo-6β-hydroxy- β- amyrin was comparable to curcumin as positive control based on the reduction of immediate early tumor antigen expression. Molecular docking by applying Autodock Vina 1 and i-GEMDOCK v 2.1 tools indicated that compound 1 gives good docking results, as determined by their fitness score and specificity. Moreover, results showed that compound 1 isolated from Pistacia integerrima precisely attached to a region where co-crystallized ligand for receptor previously existed. Our findings may explain the use of Pistacia integerrima plant extracts as an anticancer agent in folk medicine.

【 授权许可】

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