GP30 of the mycobacteriophage CASbig impairs mycobacterial adaptation during acidic stress and in macrophages

Biocell

article

WU LI¹  LEI ZHANG²  XIAOHONG XIANG³  YANZHANG LI²  TIESHAN TENG²  JIANGZHE SI²  SHUAI QIU²  JING LUO¹  HUIQIONG SUN¹  XIAOQING LI⁴  ZHIBIN WAN¹  WEI GAO¹  HANLU ZOU¹ 

[1] Key Laboratory of Regional Characteristic Agricultural Resources, College of Life Sciences, Neijiang Normal University;School of Medical Sciences, College of Medicine, Henan University;School of Pharmacy, Chongqing Medical and Pharmaceutical College;Key Laboratory of Regional Characteristic Agricultural Resources, College of Life Sciences, Neijiang Normal University, ChinaKey Laboratory of Regional Characteristic Agricultural Resources, College of Life Sciences, Neijiang Normal University

关键词: Mycobacterium smegmatis;    gp30;    CASbig;    Acidic stress;    Macrophage;   

DOI  :  10.32604/biocell.2020.011941

学科分类：仪器

来源: Biocell

【 摘 要 】

The rapid emergence of multidrug-resistant and extensively drug-resistant Tuberculosis retrieved intense interest in phage-based therapy. This old approach, which was abandoned in the west in the 1940s but is generating renewed interest, has stimulated fresh research on mycobacteriophages and their lytic efficiency against their hosts. GP30 is a novel protein of the mycobacteriophage CASbig with undiscovered function. In this study, we analyzed the role of CASbig gp30 in the host Mycobacterium smegmatis . Overexpression of gp30 in the host led to reduced growth in acidic medium and attenuated the intracellular survival rate of M. smegmatis inside the THP-1 macrophages, which may be linked to the altered lipid profile of the recombinant bacterial cell wall. In a word, this study suggested that gp30, a novel gene from a mycobacteriophage, modulated lipid composition and content to hamper the survivability of bacteria under stress conditions.

【 授权许可】

CC BY