Expression profiling of immune cells in systemic lupus erythematosus by single-cell RNA sequencing

Biocell

article

XIANLIANG HOU¹  DONGZHOU LIU⁴  YONG DAI¹  DONGE TANG¹  FENGPING ZHENG¹  MINGLIN OU³  YONG XU¹  HUIXUAN XU¹  XIAOPING HONG⁴  XINZHOU ZHANG¹  WEIER DAI⁵ 

[1] Clinical Medical Research Center, The Second Clinical Medical College, Jinan University (Shenzhen People’s Hospital);The First Affiliated Hospital, Jinan University;Central Laboratory, The Second Affiliated Hospital of Guilin Medical University;Department of Rheumatology and Immunology, the Second Clinical Medical College of Jinan University (Shenzhen People’s Hospital);College of Natural Science, University of Texas at Austin

关键词: B cell;    Differential expression genes;    Gene ontologies;    Monocytes;    Systemic lupus erythematosus;    Single-cell RNA-sequencing;   

DOI  :  10.32604/biocell.2020.011022

学科分类：仪器

来源: Biocell

【 摘 要 】

Systemic lupus erythematosus (SLE) is a systemic autoimmune disease characterized by abnormal cellular and humoral immune responses and excessive autoantibody production. The precise pathologic mechanism of SLE remains elusive. The advent of single-cell RNA sequencing (scRNA-seq) enables unbiased analysis of the molecular differences of cell populations at the single-cell level. We used scRNA-seq to profile the transcriptomes of peripheral blood mononuclear cells from an SLE patient compared with a healthy control (HC). A total of 16,021 cells were analyzed and partitioned into 12 distinct clusters. The marker genes of each cluster and the four major immune cell types (B cells, CD4+ T cells, CD8+ T cells, myeloid cells, and NK cells) were determined. Moreover, several genes involved in antigen processing and presentation through MHCII were highly enriched. GO enrichment analyses revealed abnormal gene expression patterns and signaling pathways in SLE. Of note, pseudotime analysis revealed that there was a different lineage hierarchy in the peripheral blood mononuclear cells (PBMCs) of the SLE patient, indicating that the cell states were substantially altered under disease conditions. Our analysis provides a comprehensive map of the cell types and states of the PBMCs of SLE patients at the single-cell level for a better understanding of the pathogenesis, diagnosis, and treatment of SLE.

【 授权许可】

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