MicroRNA-708 inhibits the proliferation and chemoresistance of pancreatic cancer cells

Biocell

article

Wensong LIU¹  Yunjie LU¹  Dong ZHANG¹  Longqing SHI¹  Guangchen ZU¹  Haijiao YAN²  Donglin SUN¹ 

[1] Department of Hepatobiliary Surgery, the Third Affiliated Hospital of Soochow University;Department of Oncology, the Third Affiliated Hospital of Soochow University

关键词: Chemosensitivity;    MicroRNA-708;    Pancreatic cancer;    Proliferation;    Survivin;   

DOI  :  10.32604/biocell.2020.08613

学科分类：仪器

来源: Biocell

【 摘 要 】

Pancreatic cancer is one of the most aggressive malignancies with poor prognosis and high mortality. Recent studies showed that microRNAs are dysregulated and involved in the initiation and progression of pancreatic cancer. In this study, we found that miR-708 was significantly downregulated in pancreatic cancer tissues and cell lines. Lentivirus-mediated overexpression of miR-708 could significantly inhibit the proliferation and invasion, while enhanced chemosensitivity to gemcitabine in both Panc-1 and SW1990 cells. Luciferase reporter assay showed that miR-708 bound the 3’-untranslated region of survivin and suppressed the expression of survivin in pancreatic cancer cells. In pancreatic cancer tissues, survivin protein was highly expressed and negatively correlated with miR-708 expression. Furthermore, the restoration of survivin expression could partially antagonize proliferation inhibition and apoptosis induction by miR-708 in pancreatic cancer cells. The Panc-1 cells with overexpression of miR-708 also showed decreased proliferation capability in nude mouse model compared with parental cells. In conclusion, our results suggest that miR-708 inhibits pancreatic cancer and could be a novel potential candidate to treat pancreatic cancer.

【 授权许可】

CC BY