In vitro screening of different Pseudomonas fluorescens isolates to study lytic enzyme production and growth inhibition during antagonism of Fusarium oxysporum f. sp. cumini , wilt causing pathogen of cumin

Egyptian Journal of Biological Pest Control

article

Rathore, Ridhdhi¹  Vakharia, Dinesh N.¹  Rathore, Dheeraj Singh¹ 

[1] Department of Biochemistry, College of Agriculture, Junagadh Agricultural University;Present address: Crop Science Department

关键词: Growth inhibition;    Cumin wilt;    Lytic enzymes;    Microbe-microbe interaction;    Pseudomonas;    Fusarium;   

DOI  :  10.1186/s41938-020-00259-4

学科分类：化学工程（综合）

来源: Springer

【 摘 要 】

Land plants exist in close association with bacterial and fungal microbes, where some associations can be pathogenic and others can be mutualistic/beneficial. One such relation exists between host plant, Cuminum cyminum L. (Cumin) and Fusarium oxysporum f. sp. cumini (Foc), the causal pathogen of cumin wilt and Pseudomonas fluorescens (Pf), where Pf acts as a bio-agent for inhibiting Foc and promoting plant growth of cumin. In this study, antagonism by 10 different Pf isolates against Foc was studied under laboratory conditions through percent growth inhibition and biochemical mechanisms. Among these Pf isolates, Pf-5 exhibited the highest in vitro growth inhibition (82.51%). A positive correlation was observed between percent growth inhibition and specific activities of hydrolytic enzymes, chitinase, β-1, 3 glucanase, and protease, where a negative correlation was observed with cell wall degrading enzymes, cellulase and polygalacturonase. To conclude, isolate Pf-5 could be a potential biocontrol agent for Fusarium wilt disease of cumin.

【 授权许可】

CC BY