Coating seeds with Trichoderma strains promotes plant growth and enhance the systemic resistance against Fusarium crown rot in durum wheat

Egyptian Journal of Biological Pest Control

article

Kthiri, Zayneb¹  Jabeur, Maissa Ben¹  Machraoui, Myriam¹  Gargouri, Samia²  Hiba, Khaled³  Hamada, Walid¹ 

[1] Laboratory of Genetics and Plant Breeding, National Institute of Agronomy of Tunis;National Agricultural Research Institute of Tunis;High Agronomic Institute of Chott Mariem

关键词: Seed coating;    Trichoderma sp;    Fusarium culmorum;    Wheat;    Growth promotion;    Antioxidative;    Systemic resistance;   

DOI  :  10.1186/s41938-020-00338-6

学科分类：化学工程（综合）

来源: Springer

【 摘 要 】

Fusarium crown rot is one of the major diseases that cause significant yield losses of wheat, and Trichoderma strains were known as an effective biocontrol agent. The aim of this study was to evaluate the potential of coating durum wheat seeds of the cultivar “Karim” with 3 different Tunisian strains of Trichoderma sp. (S.INAT, SIO1, SIO2) and the Trichoderma-based commercial product Trianum-T22 on seed germination, seedling growth, and plant defense response against the pathogen Fusarium culmorum. The strains were identified using molecular tools based on sequencing ITS region of ribosomal DNA. The results confirmed at 99% of homology that the strains were T. harzianum. Under controlled conditions, the coating seeds were released with 400 μl of spore suspension at 107 spores/ml. The seed coating with Trianum-P, and S.INAT showed the highest seed germination rates ranging from 85 to 90% while S.IO1 and S.IO2 presented the lowest germination rates with 66 and 68%, respectively. At 20 days post-infection (dpi) with F. culmorum, the treated plants with S.INAT and Trianum-T22 reduced the disease incidence by 53.59 and 51.79%, respectively than the control. Besides, S.INAT induced two-folds the phenolic compounds level compared to infected control. Further, the peroxidase activity was enhanced by 50% in average since 10 dpi in plants treated with S.INAT and SIO2 than the control. The results suggest that seed coating with T. harzianum S.INAT was a promising tool for crop production and protection under field conditions due to both direct antagonist activity and the indirect growth promotion. This strain seems to induce the systemic resistance of plants against foot crown rot disease.

【 授权许可】

CC BY