Association of miR-196a2 and miR-149 single-nucleotide polymorphisms with atherosclerotic ischemic stroke susceptibility

The Egyptian Journal of Neurology, Psychiatry and Neurosurgery

article

Mahmoud, Dina¹  El-Sisi, Ola¹  Sheta, Marwa¹  Ahmed, Sandra²  Fathy, Mona¹  Gomaa, Kareeman¹  Ramzy, Tarek¹ 

[1] Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University;Neurology Department, Faculty of Medicine, Cairo University

关键词: Ischemic stroke;    Micro RNAs;    miR-149;    miR-196a2;   

DOI  :  10.1186/s41983-020-00219-7

学科分类：医学（综合）

来源: Springer

【 摘 要 】

Beside common risk factors for stroke such as diabetes and hypertension, single-nucleotide variants occurring within micro RNA genes have been identified as susceptibility loci for ischemic stroke risk. Investigate the possible association of two variants in pre miRNA sequences, rs11614913 within miR-196a2 C > T and rs2292832 within miR-149 T > C, with ischemic stroke. One hundred ischemic stroke patients and 100 age and sex-matched controls having > 1 risk factor for atherosclerosis were enrolled in a case-control study. Degree of atherosclerosis was assessed using ultrasonography. Micro RNA variants were assessed by real-time PCR TaqMan probe assay. The TT genotype and T allele frequencies of miR-196a2 C > T were protective against ischemic stroke (OR 0.168, P 0.001; OR 0.482, P C variants, CC genotype was associated with increased risk by threefold (OR 3.061, P 0.005) and C allele was associated with about 1.9 fold risk of stroke (OR 1.909, P 0.002). Haplotypes analysis revealed miR-196a2T/-149 T allele combination was significantly lower among stroke patients than the controls (P < 0.001) with a protective effect (OR 0.196, 95% CI 0.083-0.466). A decrease in the incidence of ischemic stroke is associated with miR-196a2 TT genotype and T allele and increases in the likelihood risk of ischemic stroke are associated with CC genotype and C allele within miR-149, and the two miRNAs under study are closely associated with vascular damage responses.

【 授权许可】

CC BY