Pinus wallichiana -synthesized silver nanoparticles as biomedical agents: in-vitro and in-vivo approach

Green Chemistry Letters and Reviews

article

Nazish Khan¹  Ibrar Khan¹  Akhtar Nadhman²  Sadiq Azam¹  Inam Ullah¹  Farhan Ahmad³  Hamid Ali Khan⁴ 

[1] Centre of Biotechnology & Microbiology, University of Peshawar;Institute of Integrative Biosciences, CECOS University;Department of Biotechnology, Bacha Khan University;Department of Biotechnology and Microbiology, Sarhad University of Science and Technology

关键词: Pinus wallichiana;    silver nanoparticles;    Ames assay;    acute toxicity assay;    antibacterial;    antifungal;   

DOI  :  10.1080/17518253.2020.1733105

学科分类：化学（综合）

来源: Taylor & Francis

【 摘 要 】

The current study aims to assess the aqueous extract of Pinus wallichiana stem for the synthesis of small spherical-shaped (10–30 nm) silver nanoparticles (AgNPs) and their in-vitro and in-vivo biomedical applications. The biosynthesized AgNPs were nonmutagenic and safe at all test doses as per Ames and acute toxicity assay (20, 40, 60, and 80 mg/kg). The percent writhing inhibitory effect generated by AgNPs was 42.51, 50.84, and 59.06 at test doses of 10, 20, and 30 mg/kg, respectively. The percent decreased in gastrointestinal tract motility observed was 41.34%, 32.69%, and 28.48% at 10, 20, and 30 mg/kg, respectively. They also showed a significant antipyretic effect after 1, 2, and 3 h in comparison to normal saline. The AgNPs of P. wallichiana showed good antibacterial activity against Acinetobacter baumannii (60% with MIC 50  = 2.36 mg/ml and MBC = 5.0 mg/ml). These nanoparticles also possessed good antioxidant activity of 61.77 ± 0.828% and 70.25 ± 0.56% at 400 and 500 µg/ml, respectively and lack phytoagglutinin potential. Because of their high potency as biomedical agents, these nanoparticles can be a good alternative to the currently available drugs and approaches.

【 授权许可】

CC BY|CC BY-NC